0000000000901868
AUTHOR
K. Paulini
X-ray investigations of the superstructure of collagen
When compared with results from humid fibers, characteristic changes of the SAXS-pattern taken from dried collagen fibers have been observed. The average lateral width of the various reflexions increases with the order of reflexion whereby a fluctuation is superimposed, the type of which cannot easily be related to the reflexion order. Moreover, each of the reflexions is obviously laterally split into three components. An explanation of these characteristics can be given with the aid of a distinct model, assuming that the collagen fibrils have definitely varying diameters along their axis, whereby paracrystalline distortions appear, represented by nematic fluctuations. Additionally, there i…
Investigation of the superstructure of native collagen by a combination of small angle X-ray scattering, electron microscopy and light diffraction
It is shown that there exists a direct correlation between the light diffraction pattern of an electron-microscopical picture and the X-ray small angle scattering. By this means it can be proved for native collagen prepared by a special method that the density pattern of stained samples imaged in the electron microscopy is identical with the density pattern available from moist fibres. The reflex intensities of the small angle X-ray scattering are taken for calculating the density structure whereby the necessary phases are delivered by the electron microscopic examination combined with light diffraction.