0000000000924977

AUTHOR

Markus Linder

showing 2 related works from this author

Hydrophobin (HFBI): a potential fusion partner for one-step purification of recombinant proteins from insect cells

2008

Hydrophobins play an important role in binding and assembly of fungal surface structures as well as in medium-air interactions. These, hydrophobic properties provide interesting possibilities when purification of macromolecules is concerned. In aqueous micellar two-phase systems, based on surfactants, the water soluble hydrophobins are concentrated inside micellar structures and, thus, distributed to defined aqueous phases. This, one-step purification is attractive particularly when large-scale production of recombinant proteins is concerned. In the present study the hydrophobin HFBI of Trichoderma reesei was expressed as an N-terminal fusion with chicken avidin in baculovirus infected inse…

hydrophobinaqueous micellar two-phase system (AMTPS)HydrophobinRecombinant Fusion ProteinsBlotting Westernfluorescence scanning microscopy (FSM)SpodopteraMicellesurfactantslaw.inventionFungal ProteinsPulmonary surfactantlawprotein purificationProtein purificationAnimalsMicellesTrichoderma reeseiTrichodermaMicroscopy Confocalbiologytechnology industry and agricultureAvidinbiology.organism_classificationBiochemistryCytoplasmRecombinant DNAbiology.proteinBaculoviridaeBiotechnologyAvidinProtein Expression and Purification
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Biomimetic composites with enhanced toughening using silk-inspired triblock proteins and aligned nanocellulose reinforcements

2019

Silk-like proteins produced in bacteria are used as adhesives for cellulose nanofibrils to make a new biological material.

ToughnessMaterials scienceMaterials ScienceSilk02 engineering and technologymacromolecular substances010402 general chemistryProtein Engineering01 natural sciencesNanocellulosechemistry.chemical_compoundBiomimetic MaterialsSpider silkFiberCelluloseComposite materialCelluloseResearch ArticlesMultidisciplinaryCoacervatefungitechnology industry and agricultureSciAdv r-articlesLife SciencesProtein engineering021001 nanoscience & nanotechnologyequipment and suppliesRecombinant Proteins0104 chemical sciencesSILKchemistry0210 nano-technologyResearch ArticleScience Advances
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