0000000000924977
AUTHOR
Markus Linder
Hydrophobin (HFBI): a potential fusion partner for one-step purification of recombinant proteins from insect cells
Hydrophobins play an important role in binding and assembly of fungal surface structures as well as in medium-air interactions. These, hydrophobic properties provide interesting possibilities when purification of macromolecules is concerned. In aqueous micellar two-phase systems, based on surfactants, the water soluble hydrophobins are concentrated inside micellar structures and, thus, distributed to defined aqueous phases. This, one-step purification is attractive particularly when large-scale production of recombinant proteins is concerned. In the present study the hydrophobin HFBI of Trichoderma reesei was expressed as an N-terminal fusion with chicken avidin in baculovirus infected inse…
Biomimetic composites with enhanced toughening using silk-inspired triblock proteins and aligned nanocellulose reinforcements
Silk-like proteins produced in bacteria are used as adhesives for cellulose nanofibrils to make a new biological material.