0000000000925942

AUTHOR

Yannick Gueguen

showing 19 related works from this author

Characterization of MRNP34, a novel methionine-rich nacre protein from the pearl oysters

2012

9 pages; International audience; Nacre of the Pinctada pearl oyster shells is composed of 98% CaCO(3) and 2% organic matrix. The relationship between the organic matrix and the mechanism of nacre formation currently constitutes the main focus regarding the biomineralization process. In this study, we isolated a new nacre matrix protein in P. margaritifera and P. maxima, we called Pmarg- and Pmax-MRNP34 (methionine-rich nacre protein). MRNP34 is a secreted hydrophobic protein, which is remarkably rich in methionine, and which is specifically localised in mineralizing the epithelium cells of the mantle and in the nacre matrix. The structure of this protein is drastically different from those …

0106 biological sciencesBiomineralizationCalcifying mantleMethionine-richMolecular Sequence DataClinical BiochemistryGene ExpressionBiologyMatrix (biology)engineering.materialProteomics010603 evolutionary biology01 natural sciencesBiochemistryLow complexity03 medical and health sciencesPaleontologychemistry.chemical_compoundCalcification PhysiologicMethionineAnimalsAmino Acid SequencePinctada[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/BiomaterialsNacre030304 developmental biology0303 health sciencesMethionineViral matrix proteinOrganic ChemistryProteinsEpithelial Cells[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterialsbiology.organism_classificationProtein Structure TertiarychemistryBiochemistryengineeringMolluscMatrix proteinPearlBiomineralizationPinctada
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Pmarg-pearlin is a matrix protein involved in nacre framework formation in the pearl oyster Pinctada margaritifera.

2011

11 pages; International audience; The shell of pearl oysters is organized in multiple layers of CaCO(3) crystallites packed together in an organic matrix. Relationships between the components of the organic matrix and mechanisms of nacre formation currently constitute the main focus of research into biomineralization. In this study, we characterized the pearlin protein from the oyster Pinctada margaritifera (Pmarg); this shares structural features with other members of a matrix protein family, N14/N16/pearlin. Pmarg pearlin exhibits calcium- and chitin-binding properties. Pmarg pearlin transcripts are distinctively localized in the mineralizing tissue responsible for nacre formation. More s…

OysterPteriidaeMolecular Sequence Dataengineering.materialBiologyMatrix (biology)010402 general chemistry01 natural sciencesBiochemistry03 medical and health sciencesProtein structureAnimal Shellsbiology.animalAnimalsAmino Acid SequencePinctadaRNA Messenger[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/BiomaterialsMolecular Biologyglycoproteins030304 developmental biologyorganic matrix0303 health sciencesExtracellular Matrix ProteinsEcologyAragoniteOrganic ChemistryPinctada margaritiferabiology.organism_classificationbiomineralization[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterials0104 chemical sciencesCell biologyprotein structuresengineeringMolecular Medicinepearl oysterPearlBiomineralization
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Additional file 1 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Additional file 1:Table S1: Summary of BlastX results of biomineralization-related protein in the EST P. margaritifera mantle database. A catalogue of 82 P. margaritifera mantle transcripts potentially implicated in the biomineralization process was constructed using BlastX (E-value < 10-3) with selected protein sequences identified from mollusks (bivalvia and gastropoda). (DOC 140 KB)

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Characterization of molecular processes involved in the pearl formation in Pinctada margaritifera for the sustainable development of pearl farming in…

2011

11 pages; International audience

[SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/BiomaterialsPinctada margaritiferapearl oyster[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterialsbiomineralization[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/BiomaterialsComputingMilieux_MISCELLANEOUS
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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

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Additional file 3 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 2

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Characterization of molecular processes involved in the pearl formation in Pinctada margaritifera for a sustainable development of pearl farming indu…

2013

Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”. This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2) understanding pearl oyster larvae dispersal and recruitment; (3) understanding t…

Pinctada margaritiferapearl oysterbiomineralization
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Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2010

Abstract Background The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. Results We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera biomineralization-related mantle protein sequences. Component…

0106 biological sciencesModels MolecularProteomicsProteome[SDV]Life Sciences [q-bio]Proteomics01 natural sciencesContig MappingMantle (mollusc)MargaritiferaIn Situ HybridizationGeneticsExpressed Sequence Tags0303 health sciencesMineralsbiologyPinctada margaritifera[ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]ProteomeBiotechnologyResearch Articlelcsh:QH426-470Sequence analysislcsh:BiotechnologyMolecular Sequence Data010603 evolutionary biology03 medical and health sciencesCalcification Physiologiclcsh:TP248.13-248.65[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]GeneticsAnimals[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyAmino Acid SequencePinctadaRNA Messenger[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials030304 developmental biologyBase SequenceSequence Homology Amino AcidGene Expression ProfilingAnimal StructuresMolecular Sequence AnnotationSequence Analysis DNAbiology.organism_classification[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterialslcsh:GeneticsGene Expression RegulationEvolutionary biologyPinctadaBiomineralization
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'Shellome': Proteins involved in mollusk shell biomineralization - diversity, functions.

2011

18 pages; International audience

[SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterialsproteomicsnacrepearl oyster[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterials[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/BiomaterialsEST dataComputingMilieux_MISCELLANEOUSshell matrix
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Additional file 5 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 4

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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

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Additional file 4 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 3

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Additional file 2 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 1

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Additional file 5 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 4

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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

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Additional file 4 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 3

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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

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Additional file 2 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 1

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