0000000000927132

AUTHOR

Nils H. Rohleder

showing 5 related works from this author

The photoperiod entrains the molecular clock of the rat pineal.

2005

The suprachiasmatic nucleus-pineal system acts as a neuroendocrine transducer of seasonal changes in the photoperiod by regulating melatonin formation. In the present study, we have investigated the extent to which the photoperiod entrains the nonself-cycling oscillator in the Sprague-Dawley rat pineal. For this purpose, the 24-h expression of nine clock genes (bmal1, clock, per1, per2, per3, cry1, cry2, dec1 and dec2) and the aa-nat gene was monitored under light-dark 8 : 16 and light-dark 16 : 8 in the rat pineal by using real-time RT-PCR. The 24-h pattern of the expression of only per1, dec2 and aa-nat genes was affected by photoperiod. In comparison with the short photoperiod, the durat…

Maleendocrine systemmedicine.medical_specialtyTime FactorsPhotoperiodCLOCK ProteinsClockworkBiologyPineal GlandRats Sprague-DawleyBiological ClocksInternal medicinemedicineAnimalsCircadian rhythmRNA MessengerphotoperiodismAnalysis of VarianceReverse Transcriptase Polymerase Chain ReactionGeneral NeuroscienceAdaptation PhysiologicalRatsPER2CLOCKDEC1PER3EndocrinologyGene Expression RegulationTrans-ActivatorsFemaleSuprachiasmatic Nucleussense organshormones hormone substitutes and hormone antagonistsPER1The European journal of neuroscience
researchProduct

Daily oscillation of gene expression in the retina is phase-advanced with respect to the pineal gland

2007

Abstract The photoreceptive retina and the non-photoreceptive pineal gland are components of the circadian and the melatonin forming system in mammals. To contribute to our understanding of the functional integrity of the circadian system and the melatonin forming system we have compared the daily oscillation of the two tissues under various seasonal lighting conditions. For this purpose, the 24-h profiles of the expression of the genes coding for arylalkylamine N-acetyltransferase (AA-NAT), nerve growth factor inducible gene-A (NGFI-A), nerve growth factor inducible gene-B (NGFI-B), retinoic acid related orphan receptor β (RORβ), dopamine D4 receptor, and period2 (Per2) have been simultane…

Maleendocrine systemmedicine.medical_specialtyGene ExpressionCell Cycle ProteinsBiologyArylalkylamine N-AcetyltransferasePineal GlandRetinaPinealocyteRats Sprague-DawleyMelatoninPineal glandInternal medicinemedicineAnimalsCircadian rhythmMolecular BiologyEarly Growth Response Protein 1RetinaSuprachiasmatic nucleusGeneral NeuroscienceReceptors Dopamine D4Nuclear ProteinsPeriod Circadian ProteinsMicroarray AnalysisCircadian RhythmRatsPER2Endocrinologymedicine.anatomical_structureFemaleNeurology (clinical)Developmental BiologyEndocrine glandmedicine.drugBrain Research
researchProduct

Influence of photoperiodic history on clock genes and the circadian pacemaker in the rat retina

2006

The influence of seasonal lighting conditions on expression of clock genes and the circadian pacemaker was investigated in the rat retina. For this purpose, the 24-h profiles of nine clock genes (bmal1, clock, per1, per2, per3, dec1, dec2, cry1 and cry 2) and the arylalkylamine N-acetyltransferase gene as an indicator of the circadian pacemaker output were compared between light-dark periods of 8 : 16 and 16 : 8 h. The photoperiod influenced the daily patterns of the amount of transcript for per1, per3, dec2 and arylalkylamine N-acetyltransferase. This indicates that photoperiodic information modulates clock gene expression in addition to the circadian pacemaker of the retina. Under constan…

Maleendocrine systemmedicine.medical_specialtyTime FactorsPhotoperiodGene ExpressionBiologyRetinaRats Sprague-DawleyInternal medicinemedicineAnimalsRNA MessengerEye ProteinsOscillating genephotoperiodismAnalysis of VarianceReverse Transcriptase Polymerase Chain ReactionGeneral NeuroscienceNuclear ProteinsCircadian RhythmRatsPER2CLOCKDEC1PER3EndocrinologyGene Expression RegulationArylalkylamineFemalesense organshormones hormone substitutes and hormone antagonistsPER1European Journal of Neuroscience
researchProduct

Phosphodiesterase 10A in the Rat Pineal Gland: Localization, Daily and Seasonal Regulation of Expression and Influence on Signal Transduction

2010

The cyclic nucleotide phosphodiesterase 10A (PDE10A) is highly expressed in striatal spiny projection neurons and represents a therapeutic target for the treatment of psychotic symptoms. As reported previously [J Biol Chem 2009; 284:7606–7622], in this study PDE10A was seen to be additionally expressed in the pineal gland where the levels of PDE10A transcript display daily changes. As with the transcript, the amount of PDE10A protein was found to be under daily and seasonal regulation. The observed cyclicity in the amount of PDE10A mRNA persists under constant darkness, is blocked by constant light and is modulated by the lighting regime. It therefore appears to be driven by the master cloc…

Malemedicine.medical_specialtyAANATPhosphodiesterase InhibitorsEndocrinology Diabetes and MetabolismBlotting WesternBiologyReal-Time Polymerase Chain ReactionPineal GlandRats Sprague-DawleyCellular and Molecular NeurosciencePineal glandEndocrinologyOrgan Culture TechniquesInternal medicinePapaverinemedicineCyclic AMPAnimalsImmunoprecipitationProtein kinase A signalingCyclic GMPDNA PrimersPhosphodiesterase 10A;Circadian system;Pineal gland;Norepinephrine;cAMP;cGMP;Arylalkylamine N-acetyltransferaseEndocrine and Autonomic SystemsSuprachiasmatic nucleusPhosphoric Diester HydrolasesPhosphodiesteraseImmunohistochemistryCircadian RhythmRatsddc:medicine.anatomical_structureEndocrinologySecond messenger systemRNAFemaleSuprachiasmatic NucleusPDE10ASeasonsSignal transductionAdrenergic alpha-AgonistsSignal Transduction
researchProduct

Cyclic AMP-inducible genes respond uniformly to seasonal lighting conditions in the rat pineal gland

2006

The encoding of photoperiodic information ensues in terms of the daily profile in the expression of cyclic AMP (cAMP)-inducible genes such as the arylalkylamine N-acetyltransferase (AA-NAT) gene that encodes the rate-limiting enzyme in melatonin formation. In the present study, we compared the influence of the photoperiodic history on the cAMP-inducible genes AA-NAT, inducible cyclic AMP early repressor (ICER), fos-related antigen-2 (FRA-2), mitogen-activated protein kinase phosphatase-1 (MKP-1), nerve growth factor inducible gene-A (NGFI-A) and nerve growth factor inducible gene-B (NGFI-B) in the pineal gland of rats. For this purpose, we monitored the daily profiles of each gene in the sa…

Maleendocrine systemmedicine.medical_specialtyLightRepressorBiologyPineal GlandCyclic AMP Response Element ModulatorRats Sprague-DawleyPineal glandInternal medicineCyclic AMPmedicineAnimalsRNA MessengerCircadian rhythmProtein kinase AphotoperiodismReverse Transcriptase Polymerase Chain ReactionGeneral NeuroscienceCircadian RhythmRatsEndocrinologyNerve growth factormedicine.anatomical_structureGene Expression RegulationArylalkylamineFemalehormones hormone substitutes and hormone antagonistsEndocrine glandNeuroscience
researchProduct