0000000000930622
AUTHOR
A. Cebrian-serrano
showing 3 related works from this author
Beneficial Effect of Two Culture Systems with Small Groups of Embryos on the Development and Quality of In Vitro-Produced Bovine Embryos
2014
Summary Currently, in vitro-produced embryos derived by ovum pick up (OPU) and in vitro fertilization (IVF) technologies represent approximately one-third of the embryos worldwide in cattle. Nevertheless, the culture of small groups of embryos from an individual egg donor is an issue that OPU-IVF laboratories have to face. In this work, we tested whether the development and quality of the preimplantation embryos in vitro cultured in low numbers (five embryos) could be improved by the addition of epidermal growth factor, insulin, transferrin and selenium (EGF-ITS) or by the WOW system. With this aim, immature oocytes recovered from slaughtered heifers were in vitro matured and in vitro ferti…
Effect of the Bovine Oviductal Fluid onIn VitroFertilization, Development and Gene Expression ofIn Vitro-Produced Bovine Blastocysts
2012
Oviductal microenvironment generally provides better condi-tions for early embryo development than the conventionalin vitro system. In an attempt to simulate the oviductconditions or the main potentially influencing factors, theeffect was studied of a bovine oviductal fluid (bOF) treatmentapplied prior to IVF on (i) IVF parameters, (ii) cleavage rate,(iii) blastocyst yield and (iv) blastocyst quality. Embryo qualitywas assessed by morphological embryo quality and relativetranscript abundance of several developmental genes in bovineblastocysts. Furthermore, to study the effect of bOF withoutthe male effect and zona–sperm interaction, artificially acti-vated metaphase II oocytes were also treated w…
Beneficial Effect of Melatonin on Blastocyst In Vitro Production from Heat-Stressed Bovine Oocytes
2013
Melatonin may play an important role in protecting gametes and embryos from the potential harmful effects of oxidative stress. In this study, we first examined two different heat stress (HS) treatments for in vitro oocyte maturation (Experiment 1: 38.5 vs 41.0°C, during the first 20 h; Experiment 2: 38.5 vs 41.5°C, during the entire period) on bovine oocyte maturation and embryo development. Second, we tested different melatonin concentrations added to the maturation and culture medium (Experiment 3: 0, 10(-12) , 10(-9) , 10(-4) m; Experiment 4: 0, 10(-3) m), both with and without HS (38.5 or 41.5°C, respectively). In Experiment 1, the HS treatment resulted in a lower maturation rate and …