0000000000931183

AUTHOR

Linda C. Giudice

showing 2 related works from this author

Controlled Ovarian Stimulation Induces a Functional Genomic Delay of the Endometrium with Potential Clinical Implications

2008

Context: Controlled ovarian stimulation induces morphological, biochemical, and functional genomic modifications of the human endometrium during the window of implantation. Objective: Our objective was to compare the gene expression profile of the human endometrium in natural vs. controlled ovarian stimulation cycles throughout the early-mid secretory transition using microarray technology. Method: Microarray data from 49 endometrial biopsies obtained from LH+1 to LH+9 (n = 25) in natural cycles and from human chorionic gonadotropin (hCG) +1 to hCG+9 in controlled ovarian stimulation cycles (n = 24) were analyzed using different methods, such as clustering, profiling of biological processes…

medicine.medical_specialtyendocrine systemEndocrinology Diabetes and Metabolismmedia_common.quotation_subjectClinical BiochemistryStimulationLuteal PhaseBiologyEndometriumChorionic GonadotropinBiochemistryHuman chorionic gonadotropinEndometriumEndocrinologyOvulation InductionReference ValuesInternal medicinemedicineHumansMenstrual CycleMenstrual cycleOligonucleotide Array Sequence Analysismedia_commonRegulation of gene expressionGlutathione PeroxidaseGenome HumanReverse Transcriptase Polymerase Chain ReactionMicroarray analysis techniquesurogenital systemBiochemistry (medical)Luteinizing HormoneInsulin-Like Growth Factor Binding ProteinsGene expression profilingInsulin-Like Growth Factor Binding Protein 3Endocrinologymedicine.anatomical_structureGene Expression RegulationGene chip analysisRNAFemaleAlgorithms
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Transcriptomic Signature of Trophoblast Differentiation in a Human Embryonic Stem Cell Model1

2011

Identification of genes involved in trophoblast differentiation is of great interest in understanding cellular and molecular mechanisms involved in placental development and is relevant clinically to fetal development, fertility, and maternal health. Herein, we investigated differentiation of human embryonic stem cells (hESCs) down the trophoblast lineage by culture with bone morphogenetic protein 4 (BMP4) over a 10-day period. Within 2 days, the stemness markers POU5F1 and NANOG were markedly down-regulated, followed temporally by up-regulation of the CDX2, KRT7, HLA-G, ID2, CGA, and CGB trophoblast markers. To understand, on a global scale, changes in the transcriptome during the differen…

GeneticsHomeobox protein NANOGCellular differentiationWnt signaling pathwayTrophoblastCell BiologyGeneral MedicineBiologyCell biologyGene expression profilingTranscriptomemedicine.anatomical_structureReproductive Medicineembryonic structuresmedicineStem cellDevelopmental biologyreproductive and urinary physiologyBiology of Reproduction
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