Determination of methemoglobin in human blood after ionising radiation by EPR

In the present work presents results of investigations of radiation influence on blood of patients examined by radio-isotopes diagnosis (Tc 99m ), blood of Chernobyl clean-up workers and human blood irradiated by LINAC using Electron Paramagnetic Resonance (EPR). The EPR spectroscopy reveals information on electronic states of transition metal ions, particularly Fe 3+ in different spin states. It is shown that EPR spectra of blood of patients before examination has signal from metal-protein transferrin (g=4.3) and after administration of radio- isotope proves signal of Fe 3+ (methemaglobin) in the high spin state (g=6.0). The EPR spectra of Chernobyl liquidator display number of signals inc…