The recent literature about micronuclei. Consideration and reliefs.

Expression of Cyclooxygenase-1 and Cyclooxygenase-2 in normal and pathological human oral mucosa

Abstract: Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins (PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cell homeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is strongly expressed in inflammation. The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosal reactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positive outcome of oral cancer; therefore it would be useful to identify molecular markers whose expr…

Prevalence and distribution of odontogenic cysts in Sicily: 1986-2005.

The objective of this study was to assess the prevalence of odontogenic cysts (OCs) in Sicily and evaluate their distribution during a 20-year period. A cross-sectional retrospective study was carried out in 1,310 cysts of the jaw diagnosed in 12,197 individuals, who consecutively attended the Odontostomatologic Clinic of Palermo from 1986 to 2005. 1,273 cysts were classified as odontogenic, whereas only 37 were non-odontogenic. In the former group, the most frequent lesions were radicular cysts (84.5%), followed by dentigerous OCs (11.4%). Inflammatory radicular cysts were observed more in male gender, younger age at diagnosis and anterior maxilla as site of presentation. Unlike dentigerou…

Le anomalie dentarie

Multiple Supernumerary Teeth with no Other Associated Diseases or Syndromes:Case Report

La cisti dermoide del pavimento orale

Maxillary Bone Necrosis Following the Use of Formaldehyde-Containing Paste: Three Case Reports

La letteratura recente sui micronuclei. Considerazioni e rilievi The recent literature about micronuclei. Considerations and reliefs

Juvenile central ossifying fibroma of the mandible: a case report

Central ossifying fibroma is a rare fibro-osseous neoplasm in young children. It is highly aggressive and has a strong tendency to recur. We describe an 11-year-old girl with juvenile central ossifying fibromas of the mandible that caused obstruction of tooth eruption of the lower left second premolar. Oral examination revealed the presence of the primary lower left second molar and a small bony hard swelling over the left side of the mandibular body. After a computed tomographic scan, an incisional biopsy was performed, and the lesion proved to be a juvenile ossifying fibroma. Although this tumor often recurs early, and many authors suggest radical en bloc resection, we performed a more co…

Maxillary bone necrosis following the use of formaldehyde containing paste: management and case series

Paraformaldehyde is used both as a disinfectant and to devitalise inflamed pulps when local anaesthesia is ineffective. Despite the clinical benefits, paraformaldehyde is not confined to the pulp, but penetrates the dentine and is gradually released as formaldehyde. This case series describes the effects on periodontal and bone tissues of paraformaldehyde used as a devitalising or disinfectant agent.

Le Granulome éosinophile des maxillaires: une zone radiotransparente qui n'est pas toujours pathognomique d'une véritable pathologie

Biometric study about dimension of ectopic buccal maxillary canines

Maxillary bone necrosis following the use of bisphosphonates in patients with cancer: management and case series

Bisphosphonates are bone-turnover modulating drugs used to treat various diseases such as certain cancers, bone metastasis, bone- and calcium-related disorders, Paget`s disease, osteoporosis and osteopenia. Recently, several preliminary reports have reported the insurgence of osteonecrosis of the mandible and/or maxilla, associated with intravenously administered bisphosphonates. This case series describes the effects of bisphosphonates used in the treatment of certain cancers on oral bone tissues.

Le Granulome éosinophile des maxillaires: une zone radiotransparente qui n’est pas toujours pathognomonique d’une véritable pathologie.

Mandibulary Eosinophilic Granuloma: Case Series

Eosinophilic granuloma is a proliferation of Langerhans cells in the bones that most commonly involving the skeletal system. The disease generally affects children or young adults with predominance in males and it is characterized by a single or multiple skeletal lesions. This study describes a few cases of mandibular Eosinophilic Granuloma and the effects of surgical curettage of lesions

Cervico-Facial Actinomycosis: Epidemiological and clinical comments

In a retrospective investigation performed in the department of Surgical Odontostomatology of Palermo University Polyclinic from 1997-2006, the authors examined 12 cases of cervico-facial actinomycosis, taking into consideration age range, gender distribution, predisposing factors and symptoms. In concordance with reports in literature, they found that the disease was perimandibular in 65%, they also report the diagnostic methods and therapeutic approaches used in the study

Management of Primary Non-Aggressive Mandibular Giant Cell Tumor: A 14 Years' Experience

Giant cell tumor is a rare non-odontogenic lesion that represents approximately 4-5% of all primary bone tumors. Such lesions derive their actual name from the constant presence of numerous giant cells, similar to osteoclasts .All the giant cell tumors hystorically is treated with curettage. Because this conservative approach is often associated with a recurrence rate of up to 70% , aggressive resection has become the standard practice. Unfortunately ,recurrence after wide resection is about 7%. The aim of the study is to describe the 14 years single institution experience with management of primary non-aggressive giant cell tumor of the jaws bone.

Sweet's syndrome induced by removable partial denture using a CoCr alloy: case report.

Sweet's syndrome is an acute febrile neutrophilic dermatosis characterized by fever, neutrophilia and erythematous skin lesions. This disease typically occurs as a reactive event in response to infective, inflammatory or neoplastic processes. The aim of this work was to describe a rare case of Sweet's syndrome induced by dental removable partial denture using a CoCr alloy.

S2-P114 MAXILLARY BONE NECROSIS FOLLOWING THE USE OF FORMALEHYD-CONTAINING PASTE. THREE CASE REPORTS

Actinomicosi cervico-facciale

MORPHOSTRUCTURAL ANALYSIS OF HUMAN DENTAL FOLLICULAR STEM CELLS ON HIGLY POROUS BONE HYDROXYAPATITE SCAFFOLD

In this study we investigated the in vitro behaviour, morphostructure and extracellular matrix synthesis of human dental follicular stem cells (hDFSCs) isolated from human dental bud, which resulted to be positive for mesenchymal markers (CD29, CD90, CD146 and CD166) by FACS analysis. Cells were analysed by light and electronic microscopy to evaluate their biological response either at week 1, that is before differentiation, or at weeks 3-6, when they had been cultured in osteogenic medium onto a highly porous natural scaffold material (Bio-Oss). Microscopy analysis of primary culture cells showed they had a mesenchymal stem cell-like morphostructure, spindle shaped, similar to the culture …

IL NEUROFIBROMA CENTRALE SOLITARIO DELLA MANDIBOLA

Possibili profili di responsabilità nelle fistole oro-sinusali iatrogene. illustrazione casistica

Morphostructural analysis of human follicular stem cells on highly porous bone hydroxyapatite scaffold

In this study we investigated the in vitro behaviour, morphostructure and extracellular matrix synthesis of human dental follicular stem cells (hDFSCs) isolated from human dental bud, which resulted to be positive for mesenchymal markers (CD29, CD90, CD146 and CD166) by FACS analysis. Cells were analysed by light and electronic microscopy to evaluate their biological response either at week 1, that is before differentiation, or at weeks 3–6, when they had been cultured in osteogenic medium onto a highly porous natural scaffold material (Bio-Oss®). Microscopy analysis of primary culture cells showed they had a mesenchymal stem cell-like morphostructure, spindle shaped, similar to the cultur…

Informazione,consenso e alleanza terapeutica in chirurgia orale ambulatoriale

lo sviluppo della odontoiatria amplia la possibilità di interventi di chirurgia orale anche in contesto ambulatoriale; questo impone una attenta valutazione dei rischi, delle possibili complicazioni e dei vantaggi per il paziente; queste informazioni devono essere effettivamente comprese dal paziente, affinchè si costruisca un valido consenso; questa modalità di comunicazione contribuisce alla fiducia posta nel sanitario e nell’équipe terapeutica, ed è alla base della alleanza terapeutica; sotto il profilo legale, si ritiene che l’informazione, ancor prima della realizzazione del contratto di cura, sia una obbligazione vincolante per il sanitario/i. Occorre dunque sviluppare sistematicament…

Management of osteonecrosis of the jaws

Immunohistochemical and transcriptional expression of the matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa

The oral cavity is exposed to chronic or recurrent, physical and chemical trauma that could lead to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). The objective of this study is to investigate the expression and the possible changes of the two matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa samples. Normal oral mucosa samples and three different types of pathological conditions (hyperplasia, dysplasia and carcinoma) were used for this study. Immunohistochemical analysis was used to evaluate protein expression for the two enzymes, while Reverse Transcription ? Polymerase Chain Reaction (RT-PCR) was used to investigate gene expression. Image…

Immunohistochemical localization of MMP-9 and Orphanin in some sample of dental pulp.

Management of primary ameloblastoma of the jaw: a 15 years' experience.

AIM: The aim of this retrospective study was to describe our 15-year experience in the management of primary ameloblastoma of the jaw. METHODS: We investigated 26 patients who had undergone surgical treatment for a single lesion, comprising six lesions of the maxillary bone and 20 tumors of the mandible: 73% had a radiolucent multilocular lesion; 23% a unicystic lesion; and 4% had an extraosseous form of the tumor. The smaller lesions (diameter <3 cm) were treated by conservative approaches; the larger lesions required radical surgical resection. Two groups were created according to whether the patients had received conservative or aggressive surgery. RESULTS: The recurrence rate was estima…

IL GRANULOMA CENTRALE A CELLULE GIGANTI DEI MASCELLARI

Central giant cell granuloma ( CGCG ), is a fibro-osseous lesion. The majority of lesions are observed in the mandible and mainly in children and young adults, more often in females than in males. This lesion, which the pathogenesis still remains obscure, appears as radiolucency, well or ill-defined, uni or multilocular with trabeculations coursing through the lesion. Histologic study shows giant cells, fibroblastic cells, and foci of hemorrhage and osteoid tissue. On the basis of clinical, radiological and histologic features, central giant cell granulomas can be classified as "non-aggressive" or "aggressive"; the histomorphometric analysis prove a significant increase in large giant cells…

A rare case of bilateral mandibular metastatic tumor

Expression of Remodelling Enzymes (MMP-2, MMP-9) in Normal and Pathological Oral Mucosa Samples

Introduction. The oral cavity contains many structures compressed in a small area. It is exposed to chronic or recurrent, physical and chemical trauma leading to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). For a long time, our group has been studying the expression of some metalloproteinases (MMP-2, MMP-9) in healthy and pathological human oral mucosa through immunohistochemistry and RT-PCR to investigate the entity of the remodelling process in the different cases. Matherials and Methods. In this paper we investigate a sample of normal oral mucosa and four pathological samples (mucocele, fibroma, dysplasia and carcinoma). We identified the localization of MMP-2 and MMP-9 th…

In vitro Evaluation of DPSCs on Natural Bone Mineral Scaffold

COX- Expression in the inflamed gingival tissue:Immunohistochemistry,Cytochemistry and Biochemistry test.

Prostaglandins (PG) are chemical mediators for most physiological and pathological responses ,including cytoprotection of the gastric mucosa, blood coagulation,inflammation and pain (1,2,3). E2 Prostaglandins (PG2) play an important role of pathogenesis of periodontal diseases, since PG2 determines vasodilatation and increased vascular permeability, oedema and erythema.Therefore,it is not surprising to find high levels of PG2 in inflamed gingival tissues.Indeed ,in inflamed gingival tissue,PG2 levels of the Gingival Fluid (GCF) are known to be higher in periodontitis than in healthy gingiva.Moreover ,in different parodontitis the high PG2 level of the GCF is related to periodontal ligament …

Un raro caso di Sindrome di Sweet associato a manifestazioni allergiche indotte da materiali dentari : Nichel-Cromo-Cobalto