Morphostructural analysis of human follicular stem cells on highly porous bone hydroxyapatite scaffold

6533b854fe1ef96bd12ae07f

RESEARCH PRODUCT

Morphostructural analysis of human follicular stem cells on highly porous bone hydroxyapatite scaffold

Francesco Burruano Francesco Caciagli Pio Conti G. Costanzo Alessandro Caraffa Filiberto Mastrangelo L. Carone E. Nargi R. Vinci Tonino Traini Enrico Gherlone Vincenzo Salini Stefano Tetè V. Dadorante R. Ciccarelli Silvia Tortorici

subject

Scaffold dental fiollicle stem cells tissue engineering porous bone hydroxyapatite (Bio-Oss (R))Immunology Dentistry Biocompatible Materials Extracellular matrix 03 medical and health sciences dental fiollicle 0302 clinical medicine Tissue engineering Highly porous Follicular phase Humans Immunology and Allergy Cells Cultured Pharmacology Dental follicle Tissue Engineering Tissue Scaffolds business.industry Chemistry Stem Cells Cell Differentiation Fibroblasts Flow Cytometry In vitro Extracellular Matrix Cell biology Durapatite Phenotype porous bone hydroxyapatite (Bio-Oss (R))030220 oncology & carcinogenesis Microscopy Electron Scanning Stem cell business Porosity Tooth 030215 immunology

description

In this study we investigated the in vitro behaviour, morphostructure and extracellular matrix synthesis of human dental follicular stem cells (hDFSCs) isolated from human dental bud, which resulted to be positive for mesenchymal markers (CD29, CD90, CD146 and CD166) by FACS analysis. Cells were analysed by light and electronic microscopy to evaluate their biological response either at week 1, that is before differentiation, or at weeks 3–6, when they had been cultured in osteogenic medium onto a highly porous natural scaffold material (Bio-Oss®). Microscopy analysis of primary culture cells showed they had a mesenchymal stem cell-like morphostructure, spindle shaped, similar to the culture of mesenchymal stem cells derived from adult bone marrow. Also, after osteogenic differentiation, these analyses indicate typical osteoblast morphostructure and reveale a tri-dimensional organization of the cells and deposition of extracellular matrix (ECM) in close contact with biomaterial. This approach would allow to personalize the scaffold for bone tissue engineering in order to accelerate the process of osteogenesis.

year	journal	country	edition	language
2007-01-01

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