Expression and localization of MMP-9, MMP-2 and iNOS in some sample of human dental pulp orthodonticaly treated.

The recent literature about micronuclei. Consideration and reliefs.

Expression of Cyclooxygenase-1 and Cyclooxygenase-2 in normal and pathological human oral mucosa

Abstract: Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins (PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cell homeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is strongly expressed in inflammation. The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosal reactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positive outcome of oral cancer; therefore it would be useful to identify molecular markers whose expr…

Prevalence and distribution of oral mucosal non-malignant lesions in the western Sicilian population.

BACKGROUNG: The aim of the present study was to determine the prevalence of oral mucosal non-malignant lesions in the Sicilian population. In addition, we evaluated the association between each oral lesion and its risk factors. METHODS: This study analyzed a total of 2539 consecutive patients, attending the Department of Surgical, Oncological and Stomatological Disciplines of Palermo University, who were examined for the presence of various oral lesions during the period from January 2012 and February 2015. RESULTS: The sample consisted of 1330 (52%) female and 1209 (48%) male. The age ranged from 13-86 years with a mean age of 47.16 years. Among these subjects 1495 (58%), presented one or …

The significance of epidermal growth factor receptor uncommon mutations in non-small cell lung cancer: A systematic review and critical appraisal

Uncommon epidermal growth factor receptor (EGFR) mutations collectively account for 10% of EGFR mutations, harboring heterogeneous molecular alterations within exons 18-21 with clinically variable responses to EGFR tyrosine kinase inhibitors (TKIs) in advanced Non-Small Cell Lung Cancer (NSCLC) patients. In addition, with the introduction of different NGS gene approach an improvement of EGFR mutations detection was reported. Today, no specific studies have prospectively evaluated uncommon sensitizing mutations in detail and no firm standard of care has been established in the first-line setting. The aim of this comprehensive review is to critically consider the clinical role of uncommon EGF…

Multiple Supernumerary Teeth with no Other Associated Diseases or Syndromes:Case Report

La cisti dermoide del pavimento orale

Maxillary Bone Necrosis Following the Use of Formaldehyde-Containing Paste: Three Case Reports

La letteratura recente sui micronuclei. Considerazioni e rilievi The recent literature about micronuclei. Considerations and reliefs

Oral Blue Nevus: Case Report

Abstract: Aim of this research is the description of a case of palatal blue nevus and the diagnostic and therapeutic strategies to adopt when this oral lesion occurs. Nevi are benign proliferations of nevus cells either in the epithelium or in the subepithelial stroma. Blue nevus of the oral mucosa is a rare lesion. The oral blue nevi are asymptomatic and have no malignant potential. More importantly blue nevus had to be differentiated from malignant melanoma. Because is difficult to distinguish oral melanocytic nevi from melanoma we believe that any oral lesion in question should be excised and sent for histopathologic evaluation.

Expression of MMP-2 and MMP-9 in odontogenic myxoma in a child: report of a clinical case

Odontogenic myxoma (OM) is a benign, locally invasive, non-metastasizing neoplasm of the jaw bones. Despite the benign nature of these lesions, there is a high rate of recurrence and the current recommended therapy, depending on the size and behaviour of the lesion, can vary from curettage with peripheral ostectomy, segmental resection up to radical resections for more aggressive lesions. OM is a rare tumour which occurs predominantly in the third decade of life and it is rare in children. Matrix metalloproteinases (MMPs) are a family of extracellular endopeptidases responsible for the degradation and remodelling of extracellular matrix, they are known to be involved in the progression and …

The Surgical Management of Oroantral Communications: Recommendations for Routine Practice Combined Endoscopic and Intraoral Approach

An oroantral communication (OAC) is a common complication in alveolar surgery that usually occurs as a result of the extraction of maxillary posterior teeth, which do not usually resolve spontaneously. Other causes may include trauma, maxillary cysts and tumours, and infections. The practicing oral and maxillofacial surgeon treating patients with oroantral communication (OAC)/oroantral fistulas should be familiar and competent with the various treatment options available. . In most cases, surgery is performed via a Tran’s oral approach and the fistula is closed with local flaps, but the results are often unsatisfactory. Although different procedures have proved to be successful, all are pre…

Non-Syndrome Patient with Multiple Supernumerary Teeth: Case Report

Polydentia is a variation in excess in the number of the teeth. Supernumerary teeth appear, especially in the maxilla, mostly between the central incisors (mesiodens) and in molars region. These positions are followed by the maxillary fourth molars, maxillary paramolars, mandibular premolars, maxillary lateral incisors, mandibular fourth molars and maxillary premolars. Supernumerary teeth may occur in single or multiple numbers bilateral occurrence is uncommon. It is rare to find multiple supernumeraries in individuals with no other associated disease or syndrome. The purpose of this study is to present, a case with mutiple supernumerary teeth in non-syndrome patient.

Maxillary bone necrosis following the use of formaldehyde containing paste: management and case series

Paraformaldehyde is used both as a disinfectant and to devitalise inflamed pulps when local anaesthesia is ineffective. Despite the clinical benefits, paraformaldehyde is not confined to the pulp, but penetrates the dentine and is gradually released as formaldehyde. This case series describes the effects on periodontal and bone tissues of paraformaldehyde used as a devitalising or disinfectant agent.

Le Granulome éosinophile des maxillaires: une zone radiotransparente qui n'est pas toujours pathognomique d'une véritable pathologie

Biometric study about dimension of ectopic buccal maxillary canines

Maxillary bone necrosis following the use of bisphosphonates in patients with cancer: management and case series

Bisphosphonates are bone-turnover modulating drugs used to treat various diseases such as certain cancers, bone metastasis, bone- and calcium-related disorders, Paget`s disease, osteoporosis and osteopenia. Recently, several preliminary reports have reported the insurgence of osteonecrosis of the mandible and/or maxilla, associated with intravenously administered bisphosphonates. This case series describes the effects of bisphosphonates used in the treatment of certain cancers on oral bone tissues.

Le Granulome éosinophile des maxillaires: une zone radiotransparente qui n’est pas toujours pathognomonique d’une véritable pathologie.

Current and future use of virtual and augmented reality in minimal invasive oral surgery

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Child mandibular giant cell granuloma: Case report

Giant Cell Granuloma is a rare non-odontogenic lesion. Its name derives from the presence into the tumor of giant cells, similar to osteoclasts. On the basis of the biological behaviour, there are 2 variants of this tumor differentiated between aggressive and non-aggressive lesions. Treatment options reported in the literature include marginal or segmental osteotomy. If the lesion is classified as non-aggressive the marginal osteotomy (curettage) is the adequate treatment; in contrast, in presence of aggressive lesions, segmental osteotomy (en bloc resection) appears to yield the best overall outcome. The aim of the present report was to describe a case of primary Giant Cell Granuloma of th…

Mandibulary Eosinophilic Granuloma: Case Series

Eosinophilic granuloma is a proliferation of Langerhans cells in the bones that most commonly involving the skeletal system. The disease generally affects children or young adults with predominance in males and it is characterized by a single or multiple skeletal lesions. This study describes a few cases of mandibular Eosinophilic Granuloma and the effects of surgical curettage of lesions

Cervico-Facial Actinomycosis: Epidemiological and clinical comments

In a retrospective investigation performed in the department of Surgical Odontostomatology of Palermo University Polyclinic from 1997-2006, the authors examined 12 cases of cervico-facial actinomycosis, taking into consideration age range, gender distribution, predisposing factors and symptoms. In concordance with reports in literature, they found that the disease was perimandibular in 65%, they also report the diagnostic methods and therapeutic approaches used in the study

Management of Primary Non-Aggressive Mandibular Giant Cell Tumor: A 14 Years' Experience

Giant cell tumor is a rare non-odontogenic lesion that represents approximately 4-5% of all primary bone tumors. Such lesions derive their actual name from the constant presence of numerous giant cells, similar to osteoclasts .All the giant cell tumors hystorically is treated with curettage. Because this conservative approach is often associated with a recurrence rate of up to 70% , aggressive resection has become the standard practice. Unfortunately ,recurrence after wide resection is about 7%. The aim of the study is to describe the 14 years single institution experience with management of primary non-aggressive giant cell tumor of the jaws bone.

Sweet's syndrome induced by removable partial denture using a CoCr alloy: case report.

Sweet's syndrome is an acute febrile neutrophilic dermatosis characterized by fever, neutrophilia and erythematous skin lesions. This disease typically occurs as a reactive event in response to infective, inflammatory or neoplastic processes. The aim of this work was to describe a rare case of Sweet's syndrome induced by dental removable partial denture using a CoCr alloy.

Expression of gelatinases (MMP-2, MMP-9) and cyclooxygenases (COX-1, COX-2) in some benign salivary gland tumors.

Salivary gland tumors, most of which are rare benign tumors, represent a histologically heterogenous group with the greatest diversity of morphological and cellular features. The aim of this study is to analyse the expression and possible interactions between gelatinases (MMP-2, MMP-9) and cyclooxygenases (COX-1, COX-2) in some benign salivary gland tumors. We investigated the expression of gelatinases and cyclooxigenases in control salivary gland, Pleomorphic adenoma and Warthin's tumor through immunohistochemistry and Reverse Transcription – Polymerase Chain Reaction (PCR). We identified the expression of both classes of enzyme in normal samples and in the two types of pathological sampl…

S2-P114 MAXILLARY BONE NECROSIS FOLLOWING THE USE OF FORMALEHYD-CONTAINING PASTE. THREE CASE REPORTS

Actinomicosi cervico-facciale

MORPHOSTRUCTURAL ANALYSIS OF HUMAN DENTAL FOLLICULAR STEM CELLS ON HIGLY POROUS BONE HYDROXYAPATITE SCAFFOLD

In this study we investigated the in vitro behaviour, morphostructure and extracellular matrix synthesis of human dental follicular stem cells (hDFSCs) isolated from human dental bud, which resulted to be positive for mesenchymal markers (CD29, CD90, CD146 and CD166) by FACS analysis. Cells were analysed by light and electronic microscopy to evaluate their biological response either at week 1, that is before differentiation, or at weeks 3-6, when they had been cultured in osteogenic medium onto a highly porous natural scaffold material (Bio-Oss). Microscopy analysis of primary culture cells showed they had a mesenchymal stem cell-like morphostructure, spindle shaped, similar to the culture …

IL NEUROFIBROMA CENTRALE SOLITARIO DELLA MANDIBOLA

Management of primary odontogenic myxoma

BACKGROUND: This retrospective study describes our experience in the management of odontogenic myxoma. METHODS: Thirty-four patients (23 female and 11 male) with single lesions were treated. Thirty-three patients were adults (mean age 33.5 years) and one child (age 6 years). Male:female ratio was 1:2. Different surgical procedures were used to treat the tumors. 11 lesions were treated by marginal osteotomy, 12 lesions by enucleation and curettage, 11 lesions by radical treatment (segmental or block resection). The follow-up period was 5-years. RESULTS: The success rate was 73.5%. The Kaplan-Meier method was applied to examine the outcome of the treatment. Analysis showed a significant diffe…

Traditional endodontic surgery versus modern technique: a 5-year controlled clinical trial.

In this study, we compared outcomes of traditional apicoectomy versus modern apicoectomy, by means of a controlled clinical trial with a 5-year follow-up. The study investigated 938 teeth in 843 patients. On the basis of the procedure performed, the teeth were grouped in 3 groups. Differences between the groups were the method of osteotomy (type of instruments used), type of preparation of retrograde cavity (different apicoectomy angles and instruments used for root-end preparation), and root-end filling material used (gray mineral trioxide aggregate or silver amalgam). Outcome (tooth healing) was estimated after 1 and 5 years, postoperatively. Clinical success rates after 1 year were 67% (…

Possibili profili di responsabilità nelle fistole oro-sinusali iatrogene. illustrazione casistica

Morphostructural analysis of human follicular stem cells on highly porous bone hydroxyapatite scaffold

In this study we investigated the in vitro behaviour, morphostructure and extracellular matrix synthesis of human dental follicular stem cells (hDFSCs) isolated from human dental bud, which resulted to be positive for mesenchymal markers (CD29, CD90, CD146 and CD166) by FACS analysis. Cells were analysed by light and electronic microscopy to evaluate their biological response either at week 1, that is before differentiation, or at weeks 3–6, when they had been cultured in osteogenic medium onto a highly porous natural scaffold material (Bio-Oss®). Microscopy analysis of primary culture cells showed they had a mesenchymal stem cell-like morphostructure, spindle shaped, similar to the cultur…

Informazione,consenso e alleanza terapeutica in chirurgia orale ambulatoriale

lo sviluppo della odontoiatria amplia la possibilità di interventi di chirurgia orale anche in contesto ambulatoriale; questo impone una attenta valutazione dei rischi, delle possibili complicazioni e dei vantaggi per il paziente; queste informazioni devono essere effettivamente comprese dal paziente, affinchè si costruisca un valido consenso; questa modalità di comunicazione contribuisce alla fiducia posta nel sanitario e nell’équipe terapeutica, ed è alla base della alleanza terapeutica; sotto il profilo legale, si ritiene che l’informazione, ancor prima della realizzazione del contratto di cura, sia una obbligazione vincolante per il sanitario/i. Occorre dunque sviluppare sistematicament…

Management of osteonecrosis of the jaws

Immunohistochemical and transcriptional expression of the matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa

The oral cavity is exposed to chronic or recurrent, physical and chemical trauma that could lead to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). The objective of this study is to investigate the expression and the possible changes of the two matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa samples. Normal oral mucosa samples and three different types of pathological conditions (hyperplasia, dysplasia and carcinoma) were used for this study. Immunohistochemical analysis was used to evaluate protein expression for the two enzymes, while Reverse Transcription ? Polymerase Chain Reaction (RT-PCR) was used to investigate gene expression. Image…

Immunohistochemical localization of MMP-9 and Orphanin in some sample of dental pulp.

Management of primary ameloblastoma of the jaw: a 15 years' experience.

AIM: The aim of this retrospective study was to describe our 15-year experience in the management of primary ameloblastoma of the jaw. METHODS: We investigated 26 patients who had undergone surgical treatment for a single lesion, comprising six lesions of the maxillary bone and 20 tumors of the mandible: 73% had a radiolucent multilocular lesion; 23% a unicystic lesion; and 4% had an extraosseous form of the tumor. The smaller lesions (diameter <3 cm) were treated by conservative approaches; the larger lesions required radical surgical resection. Two groups were created according to whether the patients had received conservative or aggressive surgery. RESULTS: The recurrence rate was estima…

Il fibroma simmetrico del mascellare superiore .

HUMAN CERVICO-FACIAL ACTINOMYCOSIS: Actinomyces in chronic granulomatous disease, epidemiological and clinical comments

In a retrospective investigation performed in the department of Surgical Odontostomatology of Palermo University Polyclinic from 2012 to 2021, the Author examined 10 cases of cervico-facial actinomycosis, taking into consideration age range, gender distribution, predisposing factors and symptoms. In concordance with reports in literature,she found that the disease was perimandibular in 65%; she also report the diagnostic methods and therapeutic approaches used in the study

IL GRANULOMA CENTRALE A CELLULE GIGANTI DEI MASCELLARI

Central giant cell granuloma ( CGCG ), is a fibro-osseous lesion. The majority of lesions are observed in the mandible and mainly in children and young adults, more often in females than in males. This lesion, which the pathogenesis still remains obscure, appears as radiolucency, well or ill-defined, uni or multilocular with trabeculations coursing through the lesion. Histologic study shows giant cells, fibroblastic cells, and foci of hemorrhage and osteoid tissue. On the basis of clinical, radiological and histologic features, central giant cell granulomas can be classified as "non-aggressive" or "aggressive"; the histomorphometric analysis prove a significant increase in large giant cells…

The Role of Tele Dentistry During the Current Corona Virus Disease 2019 (COVID-19) And in The Possible Future Pandemic

Severe acute respiratory syndrome (SARS) coronavirus (SARSCoV) is a virus that caused the first major pandemic of the third millennium...

A rare case of bilateral mandibular metastatic tumor

Expression of Remodelling Enzymes (MMP-2, MMP-9) in Normal and Pathological Oral Mucosa Samples

Introduction. The oral cavity contains many structures compressed in a small area. It is exposed to chronic or recurrent, physical and chemical trauma leading to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). For a long time, our group has been studying the expression of some metalloproteinases (MMP-2, MMP-9) in healthy and pathological human oral mucosa through immunohistochemistry and RT-PCR to investigate the entity of the remodelling process in the different cases. Matherials and Methods. In this paper we investigate a sample of normal oral mucosa and four pathological samples (mucocele, fibroma, dysplasia and carcinoma). We identified the localization of MMP-2 and MMP-9 th…

In vitro Evaluation of DPSCs on Natural Bone Mineral Scaffold

COX- Expression in the inflamed gingival tissue:Immunohistochemistry,Cytochemistry and Biochemistry test.

Prostaglandins (PG) are chemical mediators for most physiological and pathological responses ,including cytoprotection of the gastric mucosa, blood coagulation,inflammation and pain (1,2,3). E2 Prostaglandins (PG2) play an important role of pathogenesis of periodontal diseases, since PG2 determines vasodilatation and increased vascular permeability, oedema and erythema.Therefore,it is not surprising to find high levels of PG2 in inflamed gingival tissues.Indeed ,in inflamed gingival tissue,PG2 levels of the Gingival Fluid (GCF) are known to be higher in periodontitis than in healthy gingiva.Moreover ,in different parodontitis the high PG2 level of the GCF is related to periodontal ligament …

Un raro caso di Sindrome di Sweet associato a manifestazioni allergiche indotte da materiali dentari : Nichel-Cromo-Cobalto

Strumentario chirurgico e preparazione all'intervento

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