Influence of scaffold pore size on collagen I development: A new in vitro evaluation perspective

Bone tissue engineering takes part in the complex process of bone healing by combining cells, chemical/physical signals, and scaffolds with the scaffolds providing an artificial extracellular matrix network. The role of the support template for cell activity is crucial to guide the healing process. This in vitro study compared three different poly(D,L-lactic acid) scaffolds obtained by varying the pore size generated by applying different salt leaching processes. The influence of pore dimensions on the extracellular matrix produced by human osteosarcoma-derived osteoblasts (MG63 cell line) seeded on these different materials was analyzed. This study is targeted on the intermediate stage of…