Molecular characterization and Phylogenetic analysis of Turnip mosaic virus (TuMV) in Erysimum linifolium L. in Italy

In the Summer of 2012, Erysimum linifolium L. pot plants produced at an ornamental grower in Liguria region (northern Italy), showed unusual virus-like disease of dark mottle and stripes on mauve-purple petals. A virus was mechanically transmitted from symptomatic flowers to several test plant species belonging to Chenopodiaceae and Brassicaceae families. This virus was identified as an isolated of turnip mosaic virus (TuMV) by PAS-ELISA analysis, electron microscopy negatively stained crud extracts and immuno-electron microscopy (IEM) tests. In the naturally infected E. linifolium plants, TuMV occurred alone, since any other viruses either by electron microscopy or mechanical inoculations …