0000000000963632
AUTHOR
L. Tesoriere
Anti-inflammatory effects of sicilian pistachio (pistacia vera l.) Nut in an in vitro model of human intestinal epithelium
Intestinal epithelial cells play an important role in the mucosal inflammatory response. These cells synthesize and secrete inflammatory mediators, and selectively modulate the permeability of the epithelial monolayer thus exposing immune cells to antigens. Although intestinal inflammatory response is crucial to maintain gut structural integrity and function, alteration and dysregulation of inflammatory pathways contribute to tissue damage and ulceration, and are thought to be pivotal factors in the pathogenesis of different inflammatory gut diseases [1]. The limited efficacy of conventional pharmacological therapy in the intestinal inflammatory conditions has fostered research on alternati…
All-trans to 11-cis conversion of endogenous retinoids in nuclear membrane preparations from bovine retinal pigment epithelium
not avalaible
Betanin inhibits myeloperoxidase/nitrite-mediated peroxidation of human low-density lipoprotein
BETANIN INHIBITS MYELOPEROXIDASE/NITRITE-MEDIATED PEROXIDATION OF HUMAN LOW-DENSITY LIPOPROTEIN M. Allegra, L. Tesoriere, D. Butera, A.M. Pintaudi, M.A. Livrea Dipartimento Farmacochimico Tossicologico e Biologico - Facoltà di Farmacia - Università di Palermo - Via C. Forlanini 1 - 90134 Palermo INTRODUCTION: Betanin, the betalain red pigment occurring in the Cariophillalae order plants, including cactus pear, has recently been reported to posses reducing properties and to behave as lipoperoxyl radical-scavenger in vitro (1). In addition, this phytochemical is bioavailable, accumulates in human LDL after ingestion of cactus pear fruits, and is able to protect LDL against copper-induced oxid…
IL-1b induces DNA demethylation, at genome level and in specific CpG sites of IL-6 and IL-8 genes in human intestinal epithelial cells
Inflammation is a complex physiological response that requires the activity of a sophisticated regulatory network involving the activation of specific genes for defense, tissue repair and remodeling. Although transcriptional activation has been shown to be critical in the regulation of inflammatory genes (1) the role of epigenetic phenomena in the modulation of the inflammatory response is now emerging (2). Specifically, it has been recently reported that proinflammatory stimuli induce DNA demethylation in the interleukin IL-1 promoter of human articular chondrocytes (3). IL-1 cytokine, among several proinflammatory agents, represents an essential player in the inflammatory conditions of …
Regulatory properties of nucleoside phosphotransferase from mucosa of chicken intestine
Nucleoside phosphotransferase from chicken intestinal mucosa is an associated multisubunit protein which can dissociate into components of lower molecular weight. The associated and the dissociated forms have the same substrate specificity but the first (A) shows a higher V(max) and a lower S(0.5) value than the second (B), whichever phosphate donor or nucleoside acceptor was employed. Moreover with form A the interaction coefficient in the Hill plots; as measured with a phosphate donor, varied: they always showed a higher result (about 2) than with form B (about 1). A brief preincubation at 37°C of form A modified the values both of the inactivation constant and of the enzyme kinetic param…
Purification of nucleoside phosphotransferase from mucosa of chicken intestine
The authors describe the purification procedure and some properties of a nucleoside phosphotransferase obtained from chicken intestinal mucosa, the tissue of which in preliminary studies showed the highest specific activity. Sepharose 6B chromatography and electrophoresis on polyacrylamide gel subdivided the enzyme into many forms which represent various levels of an associated multisubunit protein derived by assembly of a component at lower molecular weight. Nucleotide protectors regulate the equilibrium among these different forms, favoring the production and the stabilization of supramolecular complexes of nucleoside phosphotransferase. Similar results were obtained with enzyme purified …
Valutazione degli effetti antiossidanti del Resolvis OmegaTM in un modello di disfunzione corneale in vitro.
EPA (acido eicosapentaenoico) e DHA (acido decosaesaeoico) sono derivati metabolici degli AGE (acidi grassi essenziali) e in particolare dell'acido linolenico (omega-3)
Inhibitory effect of phosphate on nucleoside phosphotransferase from chicken intestinal mucosa
Nucleoside phosphotransferase purified from chicken intestinal mucosa is a multisubunit protein of high molecular weight regulated by many nucleotides. The adsorption of a nucleotide effector to regulatory sites favors the conversion of the enzyme to a stable form and induces a modification of the substrate site, increasing its affinity for substrate donors. We studied the effects exerted by orthophosphate on the reaction and on the stability of the enzyme. P(i) inhibits competitively nucleoside phosphotransferase activity, whichever nucleoside monophosphate is employed as donor. Nucleotide effector (d-TDP) removes entirely the inhibitory effect exerted by different concentrations of P(i). …
CCDC 941509: Experimental Crystal Structure Determination
Related Article: S. Rubino, V. Di Stefano, A. Attanzio, L. Tesoriere, M.A. Girasolo, F. Nicolò, G. Bruno, S. Orecchio, G.C. Stocco|2014|Inorg.Chim.Acta|418|112|doi:10.1016/j.ica.2014.03.028