The avidin protein family : properties of family members and engineering of novel biotin-binding protein tools

Eliöiden perimä sisältää kullekin lajille tyypillisen geneettisen tiedon ja määrittelee niiden ominaisuuksia. Lajien perimän yksityiskohtainen tarkastelu ja vertailu selvittää lajien ominaisuuksien perustaa. Suuri osa perimän sisältämistä geeneistä koodaa proteiineja, jotka ovat keskeisiä solujen rakenteille ja toiminnoille.Vesa Hytönen tarkasteli väitöskirjassaan kanan avidiini-proteiinin ja samaan geeniperheeseen kuuluvien avidiinin kaltaisten geenien (AVR) koodaamien AVR-proteiinien tuottamista ja ominaisuuksia. Avidiinin tehtävä on sitoa vesiliukoista H-vitamiinia, jota kutsutaan myös biotiiniksi. Avidiinia esiintyy erityisesti kananmunan valkuaisessa, jossa sen arvellaan toimivan puolu…

Handover performance in IEEE 802.16 mobile networks

Enhancing the performance of HSDPA communication with multipoint transmission techniques

The evolution of mobile phones, tablets and other mobile communication devices has led to a significant increase in the amount of data delivered over mobile networks. In order to satisfy the climbing data rate demands of customers, existing mobile broadband networks are being expanded with new features that allow ubiquitous and consistent networking experience. This research presents three multipoint transmission techniques for Wideband Code Division Multiple Access (WCDMA) based High-Speed Downlink Packet Access (HSDPA) communication protocol. Multiflow, High-Speed Single-Frequency Network (HS-SFN) and Multipoint-to-Point Single-Frequency Network (M2P-SFN) enable transmission of user-dedicat…

Formalin treatment increases the stability and immunogenicity of coxsackievirus B1 VLP vaccine

Type B Coxsackieviruses (CVBs) are a common cause of acute and chronic myocarditis, dilated cardiomyopathy and aseptic meningitis. However, no CVB-vaccines are available for human use. We have previously produced virus-like particles (VLPs) for CVB3 with a baculovirus-insect cell production system. Here we have explored the potential of a VLP-based vaccine targeting CVB1 and describe the production of CVB1-VLPs with a scalable VLP purification method. The developed purification method consisting of tangential flow filtration and ion exchange chromatography is compatible with industrial scale production. CVB1-VLP vaccine was treated with UV-C or formalin to study whether stability and immuno…