Vigna mungo, V. radiata and V. unguiculata plants sampled in different agronomical-ecological-climatic regions of India are nodulated by Bradyrhizobium yuanmingense

International audience; Vigna mungo, Vigna radiata and Vigna unguiculata are important legume crops cultivated in India, but little is known about the genetic resources in native rhizobia that nodulate these species. To identify these bacteria, a core collection of 76 slow-growing isolates was built from root nodules of V. mungo, V. radiata and V. unguiculata plants grown at different sites within three agro-ecological-climatic regions of India. The genetic diversity of the bacterial collection was assessed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified DNA fragments of the 16S–23S rDNA intergenic spacer (IGS) region, and the symbiotic genes nifH and nodC. One …

Impacts d'applications répétées de sulfate de cuivre sur les activités et la biodiversité microbiennes. Application à l'étude des sols viticoles

Identification and phylogenetic classification of Rhizobium species by mapped restriction site polymorphism (MRSP) analysis of PCR-amplified 16S rRNA genes: Application to the characterization of communities isolated directly from soil samples and to the bias generated by analysis of mixed-cultures or mixed-16S rDNAS

Plant phenology and genetic variability in root and nodule development strongly influence genetic structuring of Rhizobium leguminosarum biovar viciae populations nodulating pea.

Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; The symbiotic relationships between legumes and their nitrogen (N-2)-fixing bacterial partners (rhizobia) vary in effectiveness to promote plant growth according to both bacterial and legume genotype. To assess the selective effect of host plant on its microsymbionts, the influence of the pea (Pisum sativum) genotype on the relative nodulation success of Rhizobium leguminosarum biovar viciae (Rlv) genotypes from the soil populations during plant development h…

Effects of repeated applications of copper sulfate on the diversity of bacterial populations as estimated by ardra analysis

International audience

Effect of repeated applications of copper sulfate on the structure of rhizobium communities

Retama species growing in different ecological-climatic areas of northeastern Algeria have a narrow range of rhizobia that form a novel phylogenetic clade within the Bradyrhizobium genus

International audience; Sixty-seven isolates were isolated from nodules collected on roots of Mediterranean shrubby legumes Retama raetam and Retama sphaerocarpa growing in seven ecological-climatic areas of northeastern Algeria. Genetic diversity of the Retama isolates was analyzed based on genotyping by restriction fragment length polymorphism of PCR-amplified fragments of the 16S rRNA gene, the intergenic spacer (IGS) region between the 16S and 23S rRNA genes (IGS), and the symbiotic genes nifH and nodC. Eleven haplotypes assigned to the Bradyrhizobium genus were identified. Significant biogeographical differentiation of the rhizobial populations was found, but one haplotype was predomin…

Impact du cuivre sur les populations de Pseudomonas fluorescents. Variabilité génétique et capacités adaptatives

National audience

Phylogenetic comparison of symbiotic (nodC and nifH) and 16S rRNA genes in strains of Rhizobium, Mesorhizobium and Bradyrhizobium isolated from Astragalus, Oxytropis and Onobrychis spp.

Various studies reported different conclusions on the phylogenetic relationship between symbiotic (nod and nif) and chromosomal (16S rRNA) genes in rhizobia (Haukka et al, 1998). Some studies suggested that the phylogeny of nod genes could be related with the host plant (Ueda et al., 1995). In a previous study, rhizobia (44 isolates) from Astragalus, Oxytropis and Onobrychis pp have been classified into 14 distinct 16S rRNA gene types independently of their host plant and geographic origin. The majority of isolates were classified within the genus Mesorhizobium, and few within Rhizobium and Bradyrhizobium genera (Laguerre et al., 1997). The aim of the present study was to determine the dive…

Effet de la monoculture du maïs sur la diversité des populations de Rhizobium

National audience

Investigation of side-effects of repeated applications of coppersulfate on microbial activities and biodiversity in order to define bioindicator groups of soil contamination by copper

International audience

Long-term effects of crop management on Rhizobium leguminosarum biovar viciae populations.

Little is known about factors that affect the indigenous populations of rhizobia in soils. We compared the abundance, diversity and genetic structure of Rhizobium leguminosarum biovar viciae populations in soils under different crop managements, i.e., wheat and maize monocultures, crop rotation, and permanent grassland. Rhizobial populations were sampled from nodules of pea- or vetch plants grown in soils collected at three geographically distant sites in France, each site comprising a plot under long-term maize monoculture. Molecular characterization of isolates was performed by PCR-restriction fragment length polymorphism of 16S-23S rDNA intergenic spacer as a neutral marker of the genomi…

At least five rhizobial species nodulate Phaseolus vulgaris in a Spanish soil

International audience

Biological nitrogen fixation for the 21st century

The biodiversity of nitrogen-fixing organisms is huge. Taxonomic and phylogenetic research is needed to structure this diversity, to facilitate communication among scientists, and to increase our understanding of the evolution and biology of diazotrophs. Molecular tools for taxonomic and biodiversity studies of diazotrophic rhizobia, frankiae, cyanobacteria and bacilli are presented in sections 2 to 5. Sections 6 to 9 focus on problems with genus and species assignment.

Diversity of Bradyrhizobia isolated from fifteen spontaneaous legumes of fallow lands in Senegal

Genotypic characterization of Bradyrhizobium strains nodulating small Senegalese legumes by 16S-23S rRNA intergenic gene spacers and amplified fragment length polymorphism fingerprint analyses

ABSTRACT We examined the genotypic diversity of 64 Bradyrhizobium strains isolated from nodules from 27 native leguminous plant species in Senegal (West Africa) belonging to the genera Abrus , Alysicarpus , Bryaspis , Chamaecrista , Cassia , Crotalaria , Desmodium , Eriosema , Indigofera , Moghania , Rhynchosia , Sesbania , Tephrosia , and Zornia , which play an ecological role and have agronomic potential in arid regions. The strains were characterized by intergenic spacer (between 16S and 23S rRNA genes) PCR and restriction fragment length polymorphism (IGS PCR-RFLP) and amplified fragment length polymorphism (AFLP) fingerprinting analyses. Fifty-three reference strains of the different B…

Genetic Diversity of Rhizobia Isolated from the Legume Genera Astragalus, Oxytropis and Onobrychis

The legume genera Astragalus and Oxytropis belong to the tribe Galegae. These legumes are common in Arctic and Temperate climatic zones. Previous studies based on cross-inoculation experiments and numerical taxonomy (Prevost et al., 1987; Novikova et al., 1993; 1994) suggested that the rhizobia associated with these legumes were closely related. Also the legume species Onobrychis viciifolia (sainfoin, tribe Hedysareae) was effectively nodulated by rhizobia isolated from Astragalus and Oxytropis spp. (Prevost et al., 1987). Rhizobia isolated from A. sinicus were classified in a new species, Mesorhizobium huakuii, (Chen et al., 1991; Jarvis et al., 1997). Other rhizobia from Astragalus and Ox…

Compatibility of Rhizobial Genotypes within Natural Populations of Rhizobium leguminosarum Biovar viciae for Nodulation of Host Legumes

ABSTRACT Populations of Rhizobium leguminosarum biovar viciae were sampled from two bulk soils, rhizosphere, and nodules of host legumes, fava bean ( Vicia faba ) and pea ( Pisum sativum ) grown in the same soils. Additional populations nodulating peas, fava beans, and vetches ( Vicia sativa ) grown in other soils and fava bean-nodulating strains from various geographic sites were also analyzed. The rhizobia were characterized by repetitive extragenomic palindromic-PCR fingerprinting and/or PCR-restriction fragment length polymorphism (RFLP) of 16S-23S ribosomal DNA intergenic spacers as markers of the genomic background and PCR-RFLP of a nodulation gene region, nodD , as a marker of the sy…