0000000000983762
AUTHOR
Luigi Minafra
Effects of endothelial cells on breast cancer cells in a co-culture system.
Radiosensitizing effect of Curcumin loaded lipid nanoparticles in breast cancer cells
Modulation of neoplastic phenotype by Extracellular Matrix components.
"Omics" of HER2-positive breast cancer.
HER2/neu amplification/overexpression is the only somatic mutation widely considered to be a marker of disease outcome and response to treatment in breast cancer. Pathologists have made large efforts to achieve accuracy in characterizing HER2/neu status. The introduction of transtuzumab contributed to development of additional measures to identify sensitive and resistant subclasses of HER2/neu-positive tumors. In this article, we describe the latest advances in HER2/neu status diagnostic assessment and the most relevant research emerging from ‘‘Omics’’ (genomics, epigenetics, transcriptomics, and proteomics) studies on HER2/neu-positive breast cancer. A large quantity of biomarkers from dif…
The Proton-Boron Reaction Increases the Radiobiological Effectiveness of Clinical Low- and High-Energy Proton Beams: Novel Experimental Evidence and Perspectives
Protontherapy is a rapidly expanding radiotherapy modality where accelerated proton beams are used to precisely deliver the dose to the tumor target but is generally considered ineffective against radioresistant tumors. Proton-Boron Capture Therapy (PBCT) is a novel approach aimed at enhancing proton biological effectiveness. PBCT exploits a nuclear fusion reaction between low-energy protons and 11B atoms, i.e. p+11B→ 3α (p-B), which is supposed to produce highly-DNA damaging α-particles exclusively across the tumor-conformed Spread-Out Bragg Peak (SOBP), without harming healthy tissues in the beam entrance channel. To confirm previous work on PBCT, here we report new in-vitro data obtained…
Proteogenomic changes in differentianting U937
The Pvt-1 oncogene is a target of Myc and is overexpressed in human neuroblastoma cells
Study of the role of particle-particle dipole interaction in dielectrophoretic devices for biomarkers identification
A three dimensional Coupled Monte Carlo-Poisson method has been used to evaluate the impact of particle-particle dipole interactions in the equilibrium distribution of a system of uncharged polarizable particles suspended in a static liquid medium under the action of an oscillating non-uniform electric field generated by polynomial electrodes. We compare the simulated distributions with experimental ones both for micro- (MDA-MB-231 breast tumor cells) and nano-(multiwall carbon nanotubes) particles. In both cases the equilibrium distributions near the electrodes are dominated by dipole interactions which locally enhance the DEP effect and promote long particles chains.
Theoretical and experimental study of the role of cell-cell dipole interaction in dielectrophoretic devices: application to polynomial electrodes
BACKGROUND: We aimed to investigate the effect of cell-cell dipole interactions in the equilibrium distributions in dielectrophoretic devices. METHODS: We used a three dimensional coupled Monte Carlo-Poisson method to theoretically study the final distribution of a system of uncharged polarizable particles suspended in a static liquid medium under the action of an oscillating non-uniform electric field generated by polynomial electrodes. The simulated distributions have been compared with experimental ones observed in the case of MDA-MB-231 cells in the same operating conditions. RESULTS: The real and simulated distributions are consistent. In both cases the cells distribution near the elec…
Anti-oncogenic role of decorin: identification of new markers.
Fibroblast’s role in breast cancer: a proteomic approach
Fibroblasts are the major mesenchymal cell types committed to the matrix formation and renewal. Moreover they are the main source of paracrine factors that influence the growth of epithelial cells of neighbouring tissues. For these properties they may be involved in tumourigenesis, either by remodelling the tumor-associated extracellular matrix (ECM), and by the production of paracrine factors that influence the growth of carcinoma cells. Studies on fibroblasts associated to carcinomas have documented their phenotypic modifications, including abnormal migratory behaviour in vitro and growth factors altered expression(Schor & Schor, 2001). In addition, fibroblasts often recruit inflammat…
Comparative Proteomic and immunohistochemistry analyses to study alphaenolase/MBP-1 isoforms expression in breast cancer
A type I Interferon (IFN-α)-induced distal enhancer differentially regulates the P1 and P2 c-Myc promoters.
The fibroblasts: co-actors in cancer.
Decorin transfection in breast cancer cells induces proteomic modulation and downregulation of matrix proteases.
Ectopic decorin induces proteomic and cytoskeleton modulation of breast cancer cells
Gene expression profiles in irradiated cancer cells
Knowledge of the molecular and genetic mechanisms underlying cellular response to radiation may provide new avenues to develop innovative predictive tests of radiosensitivity of tumours and normal tissues and to improve individual therapy. Nowadays very few studies describe molecular changes induced by hadrontherapy treatments, therefore this field has to be explored and clarified. High-throughput methodologies, such as DNA microarray, allow us to analyse mRNA expression of thousands of genes simultaneously in order to discover new genes and pathways as targets of response to hadrontherapy. Our aim is to elucidate the molecular networks involved in the sensitivity/resistance of cancer cell …
Biological and technical background of the coelomic approach for prenatal stem cell transplantation.
An enhancer-blocker associated to a matrix attachment region (MAR) insulates the chromatin domains of the human c-Myc and Pvt-1 genes
Correlation between clinical and radiographic classification of osteoarthritis and SNPs linked to osteoarthritis susceptibility
Genomic and proteomic profiling of decorin transfected breast cancer cells.
Effects of Hypoxia on 8701-BC cancer cells.
Proteomic and genomic modulations of breast cancer cells co-cultured with endothelial cells.
Theoretical and experimental study of the kinetics of particle chains near electrodes in dielectrophoretic devices
Using a three dimensional coupled Monte Carlo-Poisson algorithm and experimental results we studied the role of the particle-particle dipole interaction on the kinetics of a system of human cells suspended in a static liquid medium under the action of an oscillating non-uniform electric field generated by polynomial electrodes. We found that the kinetics of the cells during negative/repulsive dielectrophoresis depends on the local distribution of particles. If the cells have generated long chains during positive/attractive dielectrophoresis, such chains can hinder subsequent detachment resulting in a reduction of the separation/manipulation efficiency of dielectrophoretic device. This effec…
Fibroblast’s influence on breast cancer cells.
Myeloid-specific and type I INF-induced expression of c-Myc gene promoter is mediated by a new downstream enhancer element through its interaction with PU.1 and IFNs regulatory factors
New insight on genomic and proteomic profiling of decorin-transfected breast cancer cells.
Cytoskeleton and Proteomic Changes Induced by Fibroblasts on Breast Cancer Cells
Proteomic profile of hypoxic breast cancer cells
Hematopoietic stem cell distribution in two at term anencephalic fetuses.
The extraembrionic celomic approach: evidence in ovine model.
PROTEOMIC MODULATION IN BREAST CANCER CELLS INDUCED BY MICROENVIRONMENT COMPONENTS
Proteo-genomic modulations induced on breast cancer cells by endothelial cells
Endothelial cells and normal breast epithelial cells enhance invasion of breast carcinoma cells by CXCR-4-dependent up-regulation of urokinase-type plasminogen activator receptor (uPAR, CD87) expression
Here we show the increase of invasion of three breast cancer cell lines (8701-BC, MDA-MB-231 and SKBR3) upon long-term co-incubation with culture medium of normal microvascular endothelial cells (MVEC) and normal breast epithelial cells (HB2). The enhancement of invasion relied on the interaction of microvascular endothelial cell and normal breast epithelial cell CXCL12 (SDF1) chemokine, whose expression by breast cancer cells was very low, with the cognate CXCR4 receptor of malignant cells, which resulted in over-expression of the urokinase-type plasminogen activator receptor (uPAR) on their surfaces. uPAR over-expression, showed by RT-PCR and Western blotting, was paralleled by increased …