0000000001025547
AUTHOR
Valentina Di Caro
Analisi proteomica del polline di Parietaria judaica
Ruolo delle HDAC sul silenziamento genico nell’embrione di riccio di mare
Constitutive Promoter Occupancy by the MBF-1 Activator and Chromatin Modification of the Developmental Regulated Sea Urchin α-H2A Histone Gene
The tandemly repeated sea urchin alpha-histone genes are developmentally regulated. These genes are transcribed up to the early blastula stage and permanently silenced as the embryos approach gastrulation. As previously described, expression of the alpha-H2A gene depends on the binding of the MBF-1 activator to the 5' enhancer, while down-regulation relies on the functional interaction between the 3' sns 5 insulator and the GA repeats located upstream of the enhancer. As persistent MBF-1 binding and enhancer activity are detected in gastrula embryos, we have studied the molecular mechanisms that prevent the bound MBF-1 from trans-activating the H2A promoter at this stage of development. Her…
p53-Mediated downregulation of H ferritin promoter transcriptional efficiency via NF-Y.
The tumor suppressor protein p53 triggers many of the cellular responses to DNA damage by regulating the transcription of a series of downstream target genes. p53 acts on the promoter of the target genes by interacting with the trimeric transcription factor NF-Y. H ferritin promoter activity is tightly dependent on a multiprotein complex called Bbf; on this complex NF-Y plays a major role. The aim of this work was to study the modulation of H ferritin expression levels by p53. CAT reporter assays indicate that: (i) p53 overexpression strongly downregulates the transcriptional efficiency driven by an H ferritin promoter construct containing only the NF-Y recognition sequence and that the phe…
A putative membrane protein and an ABC-transporter of Planobispora rosea stimulate antibacterial activity in Streptomyces lividans
“Epigenetics of silencing of Paracentrotus lividus α-H2A gene”
Down-regulation of early sea urchin histone H2A gene relies on cis regulative sequences located in the 5' and 3' regions and including the enhancer blocker sns.
The tandem repeated sea urchin alpha-histone genes are developmentally regulated by gene-specific promoter elements. Coordinate transcription of the five genes begins after meiotic maturation of the oocyte, continues through cleavage, and reaches its maximum at morula stage, after which these genes are shut off and maintained in a silenced state for the life cycle of the animal. Although cis regulative sequences affecting the timing and the level of expression of these genes have been characterized, much less is known about the mechanism of their repression. Here we report the results of a functional analysis that allowed the identification of the sequence elements needed for the silencing …
LA DINAMICA DELLA CROMATINA NELLA REGOLAZIONE DELL’ESPRESSIONEGENICA DELL’EMBRIONE DI RICCIO DI MARE
Role of E-selectin in the modulation of metastatic phenotype
Role of E-selectin in the modulation of metastatic phenotype
ESPRESSIONE DELLA E-SELECTINA IN CELLULE TUMORALI: POSSIBILE RUOLO NELLA MODULAZIONE DEL FENOTIPO METASTATICO?
Espressione della E-selectina in cellule tumorali: possibile ruolo nella modulazione del fenotipo metastatico
A putative membrane protein and an ABC-transporter of Planobispora rosea induce antibacterial activity of Streptomyces lividans
Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracellular medium.
Abstract Background A bacterial artificial chromosomal library of Planobispora rosea, a genetically intractable actinomycete strain, was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for the presence of genes known to be involved in the biosynthesis of antibiotics. Results One clone with a 40 kb insert showed antimicrobial activity against Gram positive bacteria. Insert sequence analysis and subcloning experiments revealed that the bioactivity was due to a 3.5 kb DNA fragment containing two open reading frames. These orfs encode two proteins with high similarity to a putative membrane protein of Streptomyces coelicolor and to the nogalamycin resis…