0000000001034286

AUTHOR

Donald Y.m. Leung

showing 2 related works from this author

Modulation of Contact Sensitivity Responses by Bacterial Superantigen

1995

Superantigens are potent modulators of the immune system, especially T cells. Therefore, we determined the influence of superantigens on the T-cell-mediated immune response, contact sensitivity. We chose the combination of staphylococcal enterotoxin B (SEB) as superantigen and 2,4-dinitrofluorbenzene (DNFB) as the contact sensitizer, because in BALB/c mice SEB reacts almost exclusively with V beta 8+ T cells, and these cells are capable of transferring contact sensitivity to DNFB from sensitized donors to naive syngeneic recipients. Pretreatment with a single intradermal injection of 50 ng SEB 24 h before DNFB exposure at the same site on the lower abdomen enhanced the induction of contact …

Lymphoid Tissue24-dinitrofluorbenzeneReceptors Antigen T-Cell alpha-betaT-LymphocytesDown-Regulationchemical and pharmacologic phenomenaDermatologyEnterotoxinDermatitis Contactcontact sensitivityBacterial superantigenBiochemistrysuperantigenProinflammatory cytokineEnterotoxinsInterferon-gammaMiceImmune systemmedicineSuperantigenAnimalsIntradermal injectionMolecular BiologySensitizationSkinAntigens BacterialMice Inbred BALB CSuperantigensbusiness.industryhemic and immune systemsCell BiologyContact sensitivitybiological factorsStaphylococcal enterotoxin Bmedicine.anatomical_structureImmunologyDinitrofluorobenzeneFemaleImmunizationbusinessJournal of Investigative Dermatology
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Cutaneous Exposure to the Superantigen Staphylococcal Enterotoxin B Elicits a T-Cell-Dependent Inflammatory Response

1996

We analyzed the impact of superantigens secreted by skin-colonizing Staphylococci on the skin and the associated lymphoid tissue following epicutaneous application and intracutaneous injection of small amounts of staphylococcal enterotoxin B (SEB). A single intracutaneous injection of 50 ng of SEB elicited a strong inflammatory response in the skin of BALB/c mice. Three to 6 h later, we observed langerhans cell activation, mast cell degranulation, vasodilation, upregulation of ICAM-1, and induction of VCAM-1 on dermal blood vessels, with vascular adhesion of granulocytes. by 12 to 24 h, cell infiltration of the dermis increased, reaching the epidermis. Among the infiltrating leukocytes, a s…

Staphylococcus aureusLangerhans cellT cellVascular Cell Adhesion Molecule-1InflammationDermatitischemical and pharmacologic phenomenaDermatologyCD8-Positive T-LymphocytesPeripheral blood mononuclear cellBiochemistryEnterotoxinsMicemedicineSuperantigenAnimalsIntradermal injectionMolecular BiologyMice Inbred BALB CSuperantigensbusiness.industryDegranulationhemic and immune systemsCell Biologybiological factorsmedicine.anatomical_structureImmunologyTumor necrosis factor alphaFemalemedicine.symptombusinessJournal of Investigative Dermatology
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