0000000001035911
AUTHOR
Roberta Segreto
QUANTIFICATION OF GENETIC VARIANTS OF CASEINS IN MILK OF GIRGENTANA GOAT BREED
In the milk of ruminants, more than 95% of proteins are synthesized by six structural genes, four caseins (alphas1, beta, alphas2 and κ-caseins) and two whey proteins (alpha-lactalbumin and beta-lactoglobulin). Goat casein genes have been found to show high polymorphism, which influences not only the quantity of casein in milk but also the structural and nutritional characteristics and technological properties of milk. The aim of this work was to separate and quantify the most common allelic variants of caseins in milk of Girgentana goat breed, a Sicilian autochthonous breed, and to evaluate the effect of casein polymorphisms on casein content. The alleles at different casein genes were det…
ASSOCIATION BETWEEN THE POLYMORPHISM AT CASEIN LOCI AND MILK FATTY ACID COMPOSITION IN GIRGENTANA GOATS
composition is an important trait for the goat dairy industry because of its influence on cheese yield and the organoleptic properties of dairy products (Chilliard et al., 2003). Goat milk is particularly rich in saturated fatty acids (SFA) whereas monounsaturated (MUFA) and polyunsaturated (PUFA) FA are less abundant (Fontecha et al., 2000).The aim of this work was to provide new data to better understand the influence of polymorphism at casein loci on fatty acid profile in Girgentana goat milk. One hundred lactating Girgentana goats, homogeneous for milk production, days of lactation, body weight and diet were used. The procedure was developed using individual raw milk samples, collected …
Quantitative determination of casein genetic variants in goat milk: Application in Girgentana dairy goat breed
The study was conducted to develop a high-performance liquid chromatographic (HPLC) method to quantify casein genetic variants (αs2-, β-, and κ-casein) in milk of homozygous individuals of Girgentana goat breed. For calibration experiments, pure genetic variants were extracted from individual milk samples of animals with known genotypes. The described HPLC approach was precise, accurate and highly suitable for quantification of goat casein genetic variants of homozygous individuals. The amount of each casein per allele was: αs2-casein A = 2.9 ± 0.8 g/L and F = 1.8 ± 0.4 g/L; β-casein C = 3.0 ± 0.8 g/L and C1 = 2.0 ± 0.7 g/L and κ-casein A = 1.6 ± 0.3 g/L and B = 1.1 ± 0.2 g/L. A good correl…
STUDY OF FATTY ACIDS PROFILE IN OVINE AND CAPRINE SICILIAN DAIRY BREED AND ASSOCIATION WITH POSSIBLE CANDIDATE GENES
In Sicilia, le razze bovine, ovine e caprine e le produzioni lattiero-casearie rappresentano una risorsa importante per l’economia del settore zootecnico. L’importanza economica del latte ovino e caprino è soprattutto legata per il primo alla sua trasformazione in prodotti lattiero-caseari, per il secondo al possibile utilisso per il consumo fresco. Per entrambe queste specie, il contenuto di grasso e proteine è importante tanto quanto la produzione di latte. L’individuazione dei geni responsabili delle caratteristiche quanti-qualitative del latte dei piccoli ruminanti permetterebbe quindi di aumentare l’efficienza del miglioramento genetico e di considerare nuovi obiettivi di selezione com…
Analysis of acetyl-CoA carboxylase gene polymorphisms in Valle del Belice dairy breeds
Acetyl-CoA carboxylase is the flux-determining enzyme in the regulation of fatty acid synthesis within animal tissues. The expression of the mammary gland isoform of this enzyme, during lactation, is regulated by the acetyl-CoA carboxylase-α (ACACA) gene. The ovine ACACA gene, with 51 coding exons, is responsible for phenotypic variability observed in milk fat content and milk fatty acids (FAs) composition. However, before attempting association analyses between this enzyme and phenotypic traits of interest, a study on the genetic variability within this locus is required. The aim of this work was to sequence the encoding region of ACACA gene in Valle del Belice dairy ewes in order to ident…