Localization of the binding site of different model tannins on the salivary PRP IB5

Localization of the binding site of different model tannins on the salivary PRP IB5. 10. european symposium on Saliva

Binding site of different tannins on a human salivary proline-rich protein evidenced by dissociative photoionization tandem mass spectrometry

Abstract The sensation of astringency is thought to originate from the interaction occurring between tannins and the salivary proline-rich proteins (PRPs). Astringency perception can be modified by the structure of tannins. Herein, we study the interactions occurring between the human salivary PRP, IB5, and three model tannins with different structure, epigallocatechin gallate and the procyanidin dimers B2 and B2 3′ O -gallate, using the coupling of mass spectrometry and VUV-synchrotron radiation. The results obtained indicate that the structure of tannins, in particular the degree of polymerization and the galloylation, does not modify the binding site on IB5 involved in the interaction.

VUV photofragmentation of protonated leucine-enkephalin peptide: partial ion yield dependence on type of sequence fragments

International audience

Ionization energy of gas phase protein cations and its dependence on charge state_and structure

Ionization energy of gas phase protein cations and its dependence on charge state_and structure. Synchrotron SOLEIL Users Meeting

Electronic spectroscopy of polypeptides in the gas phase

International audience

Oxygen K-shell spectroscopy of isolated progressively solvated peptide

Gas-phase near-edge X-ray-absorption fine structure (NEXAFS) action spectroscopy around the oxygen K-edge and mass spectrometry were employed to probe isolated substance P (SP) molecular ions, both bare and progressively solvated with 4 and 11 water molecules. Detailed mass spectra of bare and hydrated precursors are presented for the resonant photon energy of 532 eV that corresponds to O1s --> pi(amide)* core excitation, triggering resonant Auger decay and fragmentation from the ionized radical molecular system. The fragmentation pattern of doubly protonated SP hydrated with 4 water molecules clearly shows a series of abundant doubly charged backbone fragments, as well as triply charged pr…

Application of VUV synchroton radiation to proteomic and analytical mass spectrometry

Application of VUV synchroton radiation to proteomic and analytical mass spectrometry. 11. international conference on Synchroton radiation instrumentation (Sri 2012)

Localization of the binding site of different model tannins on the salivary PRP IB5

Localization of the binding site of different model tannins on the salivary PRP IB5. 3. international conference on Food oral processing: physics, physiology and psychology of eating

Astringency and the interactions between a human salivary proline-rich protein and tannins

International audience

L'influence de la nanosolvation sur la stabilité des peptides à l'étude du gaz

Localization of the tannin-binding site on the salivary PRP IB5

Localization of the tannin-binding site on the salivary PRP IB5. 10. European Symposium on Saliva

Astringency and the interaction between a human salivary proline rich-protein and tannins

VUV photodissociation of bare and nanosolvated protonated nucleotide isolated in the gas phase

International audience