0000000001051052

AUTHOR

Kristina Lindström

showing 2 related works from this author

Biological nitrogen fixation for the 21st century

1998

The biodiversity of nitrogen-fixing organisms is huge. Taxonomic and phylogenetic research is needed to structure this diversity, to facilitate communication among scientists, and to increase our understanding of the evolution and biology of diazotrophs. Molecular tools for taxonomic and biodiversity studies of diazotrophic rhizobia, frankiae, cyanobacteria and bacilli are presented in sections 2 to 5. Sections 6 to 9 focus on problems with genus and species assignment.

[SDE] Environmental SciencesBacilli[SDV]Life Sciences [q-bio]BiodiversityTECHNIQUE RFLPBACTERIETAXONOMIERhizobia03 medical and health sciencesPhylogeneticsTECHNIQUE PCRETUDE COMPARATIVEPHYLOGENIESYMBIOSEComputingMilieux_MISCELLANEOUS0303 health sciencesbiologyPhylogenetic tree030306 microbiologyEcologyLEGUMINEUSEFIXATION BIOLOGIQUE DE L'AZOTE04 agricultural and veterinary sciencesLoess plateauDIVERSITE GENETIQUEbiology.organism_classification[SDV] Life Sciences [q-bio][SDE]Environmental Sciences040103 agronomy & agriculture0401 agriculture forestry and fisheriesETUDE EXPERIMENTALETaxonomy (biology)Diazotroph
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Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples"

2011

International audience; Extracting DNA directly from micro-organisms living in soil is a crucial step for the molecular analysis of soil microbial communities. However, the use of a plethora of different soil DNA extraction protocols, each with its own bias, makes accurate data comparison difficult. To overcome this problem, a method for soil DNA extraction was proposed to the International Organization for Standardization (ISO) in 2006. This method was evaluated by 13 independent European laboratories actively participating in national and international ring tests. The reproducibility of the standardized method for molecular analyses was evaluated by comparing the amount of DNA extracted, …

Microbiology (medical)DNA BacterialMicrobiological TechniquesStandardizationSoil testRibosomal Intergenic Spacer analysis[ SDV.TOX.ECO ] Life Sciences [q-bio]/Toxicology/EcotoxicologyBiologyMicrobiologyDNA Ribosomal[ SDE ] Environmental Sciences03 medical and health sciencesRNA Ribosomal 16SMolecular BiologySoil Microbiology030304 developmental biology2. Zero hungerProtocol (science)0303 health sciences030306 microbiologyEcologybusiness.industryDNA FINGERPRINTReproducibility of ResultsDNAInter-laboratory assay15. Life on landSoil DNA extraction; Standardization; Inter-laboratory assaySoil qualityDNA FingerprintingStandardizationBiotechnologyBacterial Typing TechniquesQPCRDNA profilingSoil water[SDE]Environmental Sciencessoil DNA extraction ; standardization ; inter-laboratory assay ; DNA fingerprint ; qPCR[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/EcotoxicologybusinessSoil DNA extractionSoil microbiology
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