0000000001054382

AUTHOR

Ville Hynninen

0000-0002-4645-4468

showing 3 related works from this author

Synergistic enhancement via plasmonic nanoplate-bacteria-nanorod supercrystals for highly efficient SERS sensing of food-borne bacteria

2017

Bio-sensing techniques utilizing metallic nanoparticles as a probe have gained more and more attention and play today an important role in the detection of bacteria. To date, although several sensing materials have been tested, there is still a long way to go to achieve a fast, low-cost, ultrasensitive and multifunctional substrate suitable for a universal biosensor for detection of bacterial cells. Here, we report a novel probe design based on anisotropic plasmonic nanoparticles organized to a biocompatible 3D bio-inorganic scaffold, i.e., nanoplate-bacteria-nanorod supercrystals (NBNS) with extremely high surface-enhanced Raman spectroscopic (SERS) activity as a model of synergistic plasm…

assemblyMaterials scienceta221NanoparticleNanotechnology02 engineering and technologyrecognition of microbes010402 general chemistry01 natural sciencesBiokemia solu- ja molekyylibiologia - Biochemistry cell and molecular biologybakteeritsymbols.namesakeNanoteknologia - NanotechnologyMaterials ChemistryKemia - Chemical sciencesElectrical and Electronic EngineeringbacteriaInstrumentationPlasmonPlasmonic nanoparticlesmikrobien tunnistaminenta114SERSMetals and AlloysSubstrate (chemistry)021001 nanoscience & nanotechnologyCondensed Matter Physicschemometrics0104 chemical sciencesSurfaces Coatings and FilmsElectronic Optical and Magnetic Materialsasymmetric nanoparticlessymbolsnanoparticlesNanorodnanopartikkelit0210 nano-technologyRaman spectroscopyBiosensorRaman scattering
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SERS detection of cell surface and intracellular components of microorganisms using nano-aggregated Ag substrate

2016

Abstract The intracellular and cell surface composition and structural features of gram-positive and gram-negative bacteria were identified using near-infrared surface-enhanced Raman scattering (SERS). The structural differences of components that reside in the cell envelope are manifested by their SERS spectra, e.g. gram-negative vs. gram-positive. Silver particles were used as a SERS substrate by exploiting the existence of strong local electromagnetic fields (hot spots) within nanoscale aggregates of the particles. The aggregation of silver nanoparticles was induced by magnesium ions. These hot spots reduce the screening length of the double layer. The obtained SERS spectra showed excell…

ta221Analytical chemistry02 engineering and technology01 natural sciencesSilver nanoparticleBacterial cell structuresymbols.namesaken-acetyl-D-glucosamine (NAG)bacteriaMagnesium ionSpectroscopyn-acetylmuramic acid (NAM)Double layer (biology)ChemistrySERS010401 analytical chemistrySubstrate (chemistry)silver nano-aggregates021001 nanoscience & nanotechnology0104 chemical sciencessymbolsBiophysicsCell envelope0210 nano-technologyRaman scatteringIntracellularVibrational Spectroscopy
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Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol over…

2016

AbstractA straightforward solution-based method to modify the biofunctionality of stainless steel (SS) using heterobifunctional silane-polyethylene glycol (silane-PEG) overlayers is reported. Reduced nonspecific biofouling of both proteins and bacteria onto SS and further selective biofunctionalization of the modified surface were achieved. According to photoelectron spectroscopy analyses, the silane-PEGs formed less than 10 Å thick overlayers with close to 90% surface coverage and reproducible chemical compositions. Consequently, the surfaces also became more hydrophilic, and the observed non-specific biofouling of proteins was reduced by approximately 70%. In addition, the attachment of E…

Immobilized enzymeBiofoulingSurface PropertiesBiotin02 engineering and technologyPolyethylene glycol010402 general chemistry01 natural sciencesBacterial AdhesionArticleOverlayerPolyethylene GlycolsBiofoulingchemistry.chemical_compoundLääketieteen bioteknologia - Medical biotechnologybiofunctionalitystainless steelMultidisciplinarySilanesbiologyta114Fysiikka - Physical sciences221 Nanotechnologytechnology industry and agriculture217 Medical engineeringSilanes021001 nanoscience & nanotechnologyAvidinSilane0104 chemical scienceschemistryChemical engineering216 Materials engineeringBiotinylationbiology.proteinruostumaton teräs3111 Biomedicine0210 nano-technologyHydrophobic and Hydrophilic InteractionsAvidinProtein BindingScientific Reports
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