Effectiveness of "DNA BARCODING" approach for the molecular identification of the endangered Sicilian flora

2012

In vitro rooting and acclimatization of Sicilian Hypericum perforatum

2015

Hypericum perforatum L. is traditionally used as a medicinal plant because of different bioactive compounds with documented antidepressant and anti-inflammatory activities. Plantlets mass production with high content of these secondary metabolites has been enhanced through in vitro culture but often the process has been stopped at the multiplication phase. A study was conducted in order to set up an efficient in vitro rooting and acclimatization protocol of a H. perforatum Sicilian genotype well-adapted to south Mediterranean conditions. Aseptic nodal segments were cultured on to a Murashige and Skoog (MS) basal medium supplemented with 4.44 μM 6-benzyladenine (BA) for multiplication. Micro…