0000000001063817
AUTHOR
Dimitrios Stathis
showing 2 related works from this author
Methodology for Structured Data-Path Implementation in VLSI Physical Design: A Case Study
2022
State-of-the-art modern microprocessor and domain-specific accelerator designs are dominated by data-paths composed of regular structures, also known as bit-slices. Random logic placement and routing techniques may not result in an optimal layout for these data-path-dominated designs. As a result, implementation tools such as Cadence’s Innovus include a Structured Data-Path (SDP) feature that allows data-path placement to be completely customized by constraining the placement engine. A relative placement file is used to provide these constraints to the tool. However, the tool neither extracts nor automatically places the regular data-path structures. In other words, the relative placement f…
8-Oxo-7,8-dihydroguanine in DNA does not constitute a barrier to transcription, but is converted into transcription-blocking damage by OGG1.
2011
The common DNA base modification 8-oxo-7,8-dihydroguanine (8-oxo-G) affects the efficiency and fidelity of transcription. We constructed plasmid substrates carrying single 8-oxo-G residues, specifically positioned in the transcribed or the non-transcribed DNA strands, to investigate their effects on the expression of an EGFP reporter gene and to explore the role of base excision repair in the mechanism of transcription inhibition. We report that 8-oxo-G does not directly block transcription in cells, since a single 8-oxo-G in the transcribed DNA strand did not reduce the EGFP expression levels in repair-deficient (OGG1-null) mouse embryonic fibroblast cell lines. Rather, inhibition of trans…