0000000001069389

AUTHOR

Catharina Buschmann

showing 1 related works from this author

Generation and characterization of three monoclonal IgM antiphospholipid antibodies recognizing different phospholipid antigens.

2005

Antiphospholipid antibodies (APLs) might be involved in the pathogenesis of the antiphospholipid syndrome (APS). This study analyzes the structural characteristics of monoclonal APLs derived from patients with this disease. Patient-derived B cells were immortalized using Epstein-Barr virus transformation and subsequent fusion to the myeloma cell line CB-F7. APL-producing hybridomas were cloned to obtain cell lines producing monoclonal APL. DNA encoding the variable region of heavy and light chains of the antibodies was sequenced and analyzed regarding their usage within the V-gene family and the existence of somatic hypermutation. Binding patterns of APL to various phospholipids and beta-2-…

Malemedicine.drug_classSomatic cellMolecular Sequence DataImmunoglobulin Variable RegionSomatic hypermutationEnzyme-Linked Immunosorbent AssayBiologyMonoclonal antibodyImmunoglobulin light chainGeneral Biochemistry Genetics and Molecular BiologyCell LineMiceHistory and Philosophy of ScienceAntigenimmune system diseasesmedicineAnimalsHumansAmino Acid SequenceFramework regionPhospholipidsGlycoproteinsBase SequenceReverse Transcriptase Polymerase Chain ReactionGeneral NeuroscienceAntibodies MonoclonalMiddle AgedAntiphospholipid SyndromeMolecular biologyIsotypeComplementarity Determining RegionsImmunoglobulin Mbeta 2-Glycoprotein Ibiology.proteinAntibodies Antiphospholipidlipids (amino acids peptides and proteins)AntibodyAnnals of the New York Academy of Sciences
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