Author: Beatriz Pinto

0000000001069939

AUTHOR

Beatriz Pinto

showing 1 related works from this author

Identification and typing of food-borne Staphylococcus aureus by PCR-based techniques.

2005

Abstract The possibility of using PCR for rapid identification of food-borne Staphylococcus aureus isolates was evaluated as an alternative to the API-Staph system. A total of 158 strains, 15 S. aureus , 12 other staphylococcal species, and 131 isolates recovered from 164 food samples were studied. They were phenotypically characterized by API-Staph profiles and tested for PCR amplification with specific primers directed to thermonuclease ( nuc ) and enterotoxin ( sea to see ) genes. Disagreement between the PCR results and API-Staph identification was further assessed by the analysis of randomly amplified polymorphic DNA (RAPD) profiles obtained with three universal primers (M13, T3, and T…

DNA BacterialStaphylococcus aureusMicrococcaceaeEnterotoxinBiologymedicine.disease_causeApplied Microbiology and BiotechnologyMicrobiologyDNA RibosomalPolymerase Chain Reactionlaw.inventionMicrobiologyEnterotoxinsfluids and secretionsBacterial ProteinslawRNA Ribosomal 16SGenotypemedicineCluster AnalysisMicrococcal NucleaseTypingEcology Evolution Behavior and SystematicsPolymerase chain reactionGenes rRNASequence Analysis DNAbiology.organism_classification16S ribosomal RNAEndonucleasesMolecular biologyDNA FingerprintingRAPDBacterial Typing TechniquesRandom Amplified Polymorphic DNA TechniqueStaphylococcus aureusFood MicrobiologyNucleic Acid Amplification TechniquesSystematic and applied microbiology

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PEPhysical Sciences and Engineering
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