0000000001081175
AUTHOR
Li Feng
First Determination of 2D Speed Distribution within the Bodies of Coronal Mass Ejections with Cross-correlation Analysis
The determination of the speed of Coronal Mass Ejections (CMEs) is usually done by tracking brighter features (such as the CME front and core) in visible light coronagraphic images and by deriving unidimensional profiles of the CME speed as a function of altitude or time. Nevertheless, CMEs are usually characterized by the presence of significant density inhomogeneities propagating outward with different radial and latitudinal projected speeds, resulting in a complex evolution eventually forming the Interplanetary CME. In this work, we demonstrate for the first time how coronagraphic image sequences can be analyzed with cross-correlation technique to derive 2D maps of the almost instantaneo…
Expression and characterization of the recombinant juvenile hormone epoxide hydrolase (JHEH) from Manduca sexta.
The cDNA of the microsomal Juvenile Hormone Epoxide Hydrolase (JHEH) from Manduca sexta was expressed in vitro in the baculovirus system. In insect cell culture, the recombinant enzyme (Ms-JHEH) was produced at a high level (100 fold over background EH catalytic activity). As expected, Ms-JHEH was localized in the microsomal fraction with a molecular mass of approximately 50 kDa. Ms-JHEH showed a substrate and inhibitor spectrum similar to the wild type JHEH isolated from eggs of M. sexta. Its enzymatic activity was the highest for Juvenile Hormone III. Ms-JHEH hydrolyzed several trans-epoxides faster than cis-epoxides. A putative hydroxyl-acyl enzyme intermediate was isolated suggesting a …