A whole-transcriptome approach to unravel non-target-site-based resistance to ALS inhibitors in Ambrosia artemisiifolia

International audience; Non-target-site-resistance (NTSR) to herbicides is a major cause for chemical control failure in a number of weeds. Studies investigating NTSR essentially considered grass weeds. Here, we investigated the transcriptomic bases of NTSR to ALS inhibitors in the global, allergenic weed Ambrosia artemisiifolia. Our objectives were to establish a transcriptome resource for A. artemisiifolia and to use a comparative, quantitative transcriptome sequencing approach (RNA-Seq) to identify candidate NTSR genes in this species. Using the PacBio sequencing technology that generate long sequence reads, a transcriptome resource was obtained from the apical part of one 4-leaf plant, …