0000000001097991

AUTHOR

Siroos Jafari

showing 1 related works from this author

Single tube real time PCR for detection of Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella pneumophila from …

2012

We designed a multiplex real time PCR for rapid, sensitive and specific detection of Streptococcus pneumoniae, Legionella pneumophila, Chlamydophila pneumoniae and Mycoplasma pneumoniae. The study cases consisted of 129 patients with community acquired pneumonia (CAP). Bacteriological techniques were implemented for detection of the cultivable organisms. DNA were extracted from sputa, throat swabs, bronchoalveolar lavages and tracheal aspirates and used as templates in real time PCR. The primers and probes were designed for cbpA (S. pneumoniae), p1adhesin (M. pneumoniae), mip (L. pneumophila) and ompA (C. pneumoniae). After optimization of real time PCR for every organism, the experiments w…

Settore MED/07 - Microbiologia E Microbiologia ClinicaMycoplasma pneumoniaemedicine.disease_causeReal-Time Polymerase Chain ReactionLegionella pneumophilaSensitivity and SpecificityMicrobiologyLegionella pneumophilaCommunity-acquired pneumoniacommunity acquired pneumonia CAP real time PCR Streptococcus pneumonia Legionella pneumophila Chlamydophila pneumonia Mycoplasma pneumoniaeStreptococcus pneumoniaeMultiplex polymerase chain reactionmedicinePneumonia BacterialHumansMultiplexGeneral Immunology and MicrobiologybiologyBacteriaGeneral MedicineChlamydophila pneumoniaebiology.organism_classificationmedicine.diseaseVirologyrespiratory tract diseasesMycoplasma pneumoniaeCommunity-Acquired InfectionsReal-time polymerase chain reactionStreptococcus pneumoniaeChlamydophila pneumoniaeMultiplex Polymerase Chain ReactionActa microbiologica et immunologica Hungarica
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