0000000001117709

AUTHOR

Laura Murcia

showing 2 related works from this author

Evaluation of changes in ion release and biological properties of NeoMTA‐Plus and Endocem‐MTA exposed to an acidic environment

2018

AIM To analyse in vitro changes in ion release and biological properties of Endocem-MTA (Maruchi, Wonju, Korea) and NeoMTA-Plus (Avalon Biomed Inc, Bradenton, FL, USA) exposed to acidic or neutral environment on human dental periodontal ligament stem cells (hPDLSCs). METHODOLOGY Cell viability and wound healing assays were performed using eluates of each material. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy. The chemical composition of the materials was determin…

IonsProgrammed cell deathmedicine.diagnostic_testPeriodontal ligament stem cellsSilicatesOxidesPemetrexedCalcium CompoundsFlow cytometryRoot Canal Filling MaterialsButyric acidDrug Combinationschemistry.chemical_compoundchemistryApoptosisMaterials TestingRepublic of KoreamedicineHumansMTT assayViability assayAluminum CompoundsCytotoxicityGeneral DentistryNuclear chemistryInternational Endodontic Journal
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Biological Effects of New Hydraulic Materials on Human Periodontal Ligament Stem Cells.

2019

Background: The aim of this study was: to evaluate the biological properties of new hydraulic materials: Bio-C Repair and Bio-C Sealer. Methods: Periodontal ligament stem cells were exposed to several dilutions of Bio-C Repair and Bio-C Sealer. The ion release profile and pH were determined. Metabolic activity, cell migration and cell survival were assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), wound-healing assays and Annexin assays, respectively. Cells were cultured in direct contact with the surface of each material. These were then analyzed via scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). Statistical differences were ass…

hydraulic cementsSerial dilutionPeriodontal ligament stem cellsScanning electron microscopelcsh:Medicine02 engineering and technologyOdontologiaArticle03 medical and health sciences0302 clinical medicineAnnexinMedicineViability assayCell adhesionCytotoxicityendodontic cementsbusiness.industrylcsh:Rhuman periodontal ligament stem cellsCell migration030206 dentistryGeneral Medicine021001 nanoscience & nanotechnologyMolecular biologybioceramicscytotoxicity0210 nano-technologybusinessJournal of clinical medicine
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