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RESEARCH PRODUCT

Biological Effects of New Hydraulic Materials on Human Periodontal Ligament Stem Cells.

José M. MoraledaLaura MurciaFrancisco Javier Rodríguez-lozanoAdrián LozanoLeopoldo FornerJulia Guerrero-gironésDavid García-bernalMari Carmen LlenaSergio López-garcía

subject

hydraulic cementsSerial dilutionPeriodontal ligament stem cellsScanning electron microscopelcsh:Medicine02 engineering and technologyOdontologiaArticle03 medical and health sciences0302 clinical medicineAnnexinMedicineViability assayCell adhesionCytotoxicityendodontic cementsbusiness.industrylcsh:Rhuman periodontal ligament stem cellsCell migration030206 dentistryGeneral Medicine021001 nanoscience & nanotechnologyMolecular biologybioceramicscytotoxicity0210 nano-technologybusiness

description

Background: The aim of this study was: to evaluate the biological properties of new hydraulic materials: Bio-C Repair and Bio-C Sealer. Methods: Periodontal ligament stem cells were exposed to several dilutions of Bio-C Repair and Bio-C Sealer. The ion release profile and pH were determined. Metabolic activity, cell migration and cell survival were assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), wound-healing assays and Annexin assays, respectively. Cells were cultured in direct contact with the surface of each material. These were then analyzed via scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). Statistical differences were assessed using a two-way ANOVA (&alpha

yearjournalcountryeditionlanguage
2019-08-14Journal of clinical medicine
10.3390/jcm8081216https://pubmed.ncbi.nlm.nih.gov/31416236