Chromatin structure of the Arabidopsis thaliana Adh promoter: analysis of nucleosome positioning

Abstract The chromatin structure of the promoter and proximal 5′ transcribed region of the Arabidopsis thaliana Adh gene has been studied in three experimental models: whole plants under aerobic conditions in which the gene is repressed, whole plants under flooding-induced anaerobiosis, in which the expression of the gene occurs in some plant tissues and yeast cells in which the Arabidopsis Adh DNA had been cloned but is not expressed at all. Experiments of indirect end labelling after DNase I and micrococcal nuclease digestion of nuclei allowed us to conclude that no positioned nucleosomes exist in plant nuclei on the proximal region of the promoter (up to −350), probably due to the presen…