0000000001133749
AUTHOR
Alexandre Guillaubey
An inflammatory model of dry eye syndrome using scopolamine
International audience
Time Course of Inflammation and Apoptosis in a Scopolamine–Induced Dry Eye Rat Model
International audience; Purpose: : Dry eye syndrome is due to tear secretion deficiency or excessive tear evaporation leading to tear film instability. The aim of this study was to evaluate the time course of inflammation and apoptosis in a scopolamine–induced dry eye rat model. Methods: : Osmotic pumps continuously delivering scopolamine (12.5mg/d) were implanted subcutaneously in female Lewis rats for 1, 2, 3, 7, 10, 17 and 28 days. During the treatment, the corneal status was assessed by fluorescein impregnation and tears were collected for prostaglandin E2 (PGE2) immunoassay. At the end of the treatment period, the animals were sacrificed and the ocular tissues and the lacrimal glands w…
Effect of intravitreal injection of Indocyanine Green, Triamcinolone Acetonide and Trypan Blue on the electroretinographic response of rats
International audience; Purpose:: Indocyanine Green (ICG) and Tripan Blue (TB) are commonly used in macular surgery by making easier the internal limiting membrane (ILM) removing. However, the innocuousness of these dyes is not actually established notably for ICG. Then, several authors proposed the use of triamcinolone acetonide (TA) as an alternative to the use of intraoperative dyes for visualizing the ILM. The purpose of this study was to evaluate the effects of intravitreal injection of ICG, TB and TA on the rat electroretinogram (ERG). Methods:: Three groups of 12-week-old Sprague Dawley rats (n=6) received intravitreal injection in one eye of 0.1mL of either ICG 0.5mg/mL, TB 3mg/mL o…
Evaluation of an experimental model of dry eye in the rat
International audience
Retinal Detachments Following Successful Idiopathic Macular Holes Surgery
Purpose: : To evaluate long term outcome of successfully operated idiopathic macular holes (IMH).Methods: : We conducted a comparative retrospective case series in two academic centers. IMH were operated with pars plana vitrectomy, intravitreal gas and internal limiting membrane peeling between 2002 and 2005. All these macular holes were analyzed with Stratus OCT before and after the surgery. We divided them into 4 groups: group 1: vitrectomy in pseudophakic eyes; group 2: vitrectomy and cataract extraction as a combined procedure; group 3: vitrectomy followed by cataract extraction; group 4: vitrectomy in pseudophakic eyes. We noted the anatomical and functional results and we studied seri…
Docosahexaenoic acid and ether-lipid changes and ERG alterations during retinal ischemia-reperfusion in the rat.
International audience
A detailed analysis of retinal and retinal pigment epithelium phospholipid species: focus on ether-lipids
International audience
Docosahexaenoic acid and ether-lipids are correlated to ERG alterations during retinal ischemia-reperfusion in the rat
International audience