La métaprotéomique pour l'analyse fonctionnelle des communautés bactéreinnes indigènes

Metaproteomics: a new approach for studying functional microbial ecology

Establishing the link between microbial diversity and ecosystem functioning constitutes one of the fundamental questions in microbial ecology. However, microbial biodiversity cannot be resolved in a single manner and the question arises about which of the genetic and/or taxonomic and/or functional components of biodiversity are the most relevant in such a problematic. To date, most researches in this field has focused on genetic diversity and few investigations have examined functional diversity, resulting in a poor understanding of the manner in which genetic and taxonomic diversity affect functional diversity or ecosystem properties. This can be explained by paucity of methods allowing an…

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1

Contains fulltext : 232759.pdf (Publisher’s version ) (Closed access) In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to…

Protein and DNA fingerprinting of a soil bacterial community inoculated into three different sterile soils

The functional and genetic structures of a soil bacterial community were characterized after inoculation into three different sterile soils using a protein and DNA fingerprinting method, respectively. Principal component analysis (PCA) of profiles revealed that, depending on soil characteristics, bacterial communities with similar genetic structures harbored different functional structures and thus could potentially be of differing ecological significance for soil functioning. Co-inertia analysis between protein fingerprinting data and the corresponding sets of soil physicochemical characteristics demonstrated the correlation between the functional structure of the bacterial community and s…