Design principles of chemiluminescence (CL) chemodosimeter for self-signaling detection: Luminol protective approach

Chemiluminescence (CL) sensors can provide convenience and high sensitivity because they do not require an external excitation light source to produce a fluorescence signal. However, most CL based detection systems do not have a built-in self-signaling process, leading to inefficient and complex protocols due to the required multistep cascade reactions. Here, we develop a CL based sensory system with a built-in self-signaling feature by adapting the chemodosimeter concept. We found that a masking group incorporated to luminol efficiently suppresses the CL of luminol and that selective removal of the masking group by a target analyte can turn on the CL process, generating a sensitive fluores…