0000000001158808

AUTHOR

G Juan

showing 3 related works from this author

Oxidative metabolism in a rat hepatoma (N13) and isolated rat hepatocytes: A flow cytometric comparative study

1996

Recently, we have developed a new and fast kinetic method for assessing mitochondrial membrane potential by flow cytometry, based on the quantitation of the initial rate of rhodamine 123 (Rh123) uptake by living cells. This test has proved suitable to detect metabolic and toxic effects on mitochondria. To characterize energy metabolism in a rat hepatoma cell line (N13), we applied this method to assess several metabolic pathways that eventually generate mitochondrial membrane potential. Using this approach, we found that N13 hepatoma cells retain an oxidative capacity comparable with that observed in isolated hepatocytes under the same conditions. These results show that this cell line may …

MaleOrnithineLiver cytologySuccinic AcidOleic AcidsMitochondrionBiologyRhodamine 123Flow cytometrychemistry.chemical_compoundLiver Neoplasms ExperimentalAmmoniaCarnitinemedicineAnimalsRhodamine 123Rats WistarHepatologymedicine.diagnostic_testRhodaminesSuccinatesFlow CytometryIn vitroRatsMetabolic pathwayGlucosemedicine.anatomical_structureLiverBiochemistrychemistryCell cultureHepatocyteGlycolysisOxidation-ReductionOleic AcidHepatology
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Aging of the liver: Age-associated mitochondrial damage in intact hepatocytes

1996

Mitochondrial damage may be a major cause of cellular aging. So far, this hypothesis had only been tested using isolated mitochondria. The aim of this study was to investigate the involvement of mitochondria in aging using whole liver cells and not isolated mitochondria only. Using flow cytometry, we found that age is associated with a decrease in mitochondrial membrane potential (30%), an increase in mitochondrial size, and an increase in mitochondrial peroxide generation (23%). Intracellular peroxide levels were also increased. The number of mitochondria per cell and inner mitochondrial membrane mass did not change. Gluconeogenesis from glycerol or fructose (mitochondrial-independent) did…

medicine.medical_specialtyHepatologyMitochondrionBiologyMitochondrial SizePyruvate carboxylaseEndocrinologyMitochondrial permeability transition poreGluconeogenesisInternal medicinemedicinesense organsATP–ADP translocaseInner mitochondrial membranePhosphoenolpyruvate carboxykinaseHepatology
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Cigarette smoke exposure up-regulates endothelin receptor B in human pulmonary artery endothelial cells: molecular and functional consequences

2010

BACKGROUND AND PURPOSEPulmonary arteries from smokers and chronic obstructive pulmonary disease patients show abnormal endothelium-dependent vascular reactivity. We studied the effect of cigarette smoke extract (CSE) on endothelin receptor B (ETB) expression in human pulmonary artery endothelial cells (HPAECs) and its role in endothelial dysfunction.EXPERIMENTAL APPROACHETB receptor expression was measured by real time RT-PCR, Western blot and immunofluorescence. Cell contraction, intracellular Ca2+, F/G-actin, RhoA activity, myosin light chain phosphorylation, ET, NO, thromboxane (Tx)A2 and reactive oxygen species (ROS) were measured by traction microscopy, fluorescence microscopy, phalloi…

MalePulmonary ArteryNitric OxideTransfectionMuscle Smooth Vascularendothelial dysfunctionendothelin receptor Bpulmonary artery endothelial cellsSmokeTobaccoHumansRNA Small InterferingCells CulturedAgedSulfonamidesrho-Associated KinasesEndothelin-1bosentancigarette smokeEndothelial CellsResearch PapersReceptor Endothelin BUp-RegulationThromboxane B2FemaleEndothelium VascularReactive Oxygen Species
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