6533b7d7fe1ef96bd126845a

RESEARCH PRODUCT

Oxidative metabolism in a rat hepatoma (N13) and isolated rat hepatocytes: A flow cytometric comparative study

G JuanRobert C. CallaghanRosario Gil-bensoJosé-enrique O'connor

subject

MaleOrnithineLiver cytologySuccinic AcidOleic AcidsMitochondrionBiologyRhodamine 123Flow cytometrychemistry.chemical_compoundLiver Neoplasms ExperimentalAmmoniaCarnitinemedicineAnimalsRhodamine 123Rats WistarHepatologymedicine.diagnostic_testRhodaminesSuccinatesFlow CytometryIn vitroRatsMetabolic pathwayGlucosemedicine.anatomical_structureLiverBiochemistrychemistryCell cultureHepatocyteGlycolysisOxidation-ReductionOleic Acid

description

Recently, we have developed a new and fast kinetic method for assessing mitochondrial membrane potential by flow cytometry, based on the quantitation of the initial rate of rhodamine 123 (Rh123) uptake by living cells. This test has proved suitable to detect metabolic and toxic effects on mitochondria. To characterize energy metabolism in a rat hepatoma cell line (N13), we applied this method to assess several metabolic pathways that eventually generate mitochondrial membrane potential. Using this approach, we found that N13 hepatoma cells retain an oxidative capacity comparable with that observed in isolated hepatocytes under the same conditions. These results show that this cell line may represent an adequate biological model to perform metabolic and toxicological studies in vitro.

yearjournalcountryeditionlanguage
1996-08-01Hepatology
https://doi.org/10.1002/hep.510240216