[23] Ratio of reduced to oxidized glutathione as indicator of oxidative stress status and DNA damage

Publisher Summary This chapter discusses the ratio of reduced to oxidized glutathione (GSH) as an indicator of oxidative stress status and DNA damage. Several methods have been proposed for the determination of GSH status in biological samples. Accurate determination of this status is largely dependent on the prevention of GSH autoxidation during sample processing. As the disulfide form (GSSG) is present only in minimal amounts with respect to the reduced form, a small GSH autoxidation during sample processing can give erroneously high GSSG level. The chapter describes high-performance liquid chromatography (HPLC) method for determining GSSG. It also presents a method for glutathione determ…