0000000001173606

AUTHOR

Helge Küster

showing 3 related works from this author

Construction and validation of cDNA-based Mt6k-RIT macro- and microarrays to explore root endosymbioses in the model legume Medicago truncatula

2004

To construct macro- and microarray tools suitable for expression profiling in root endosymbioses of the model legume Medicago truncatula, we PCR-amplified a total of 6048 cDNA probes representing genes expressed in uninfected roots, mycorrhizal roots and young root nodules [Nucleic Acids Res. 30 (2002) 5579]. Including additional probes for either tissue-specific or constitutively expressed control genes, 5651 successfully amplified gene-specific probes were used to grid macro- and to spot microarrays designated Mt6k-RIT (M. truncatula 6k root interaction transcriptome). Subsequent to a technical validation of microarray printing, we performed two pilot expression profiling experiments usin…

0106 biological sciencesRoot nodule[SDV]Life Sciences [q-bio]Plant Roots01 natural sciencesApplied Microbiology and BiotechnologyTranscriptomeADNCGene Expression Regulation PlantGene Expression Regulation FungalMycorrhizaeMedicagoPCR-basedComputingMilieux_MISCELLANEOUSOligonucleotide Array Sequence AnalysisPlant ProteinsExpressed Sequence Tags2. Zero hunger0303 health sciencesnodulin genesroot nodule symbiosisarbuscular mycorrhizafood and beveragesEquipment DesignGeneral MedicineMedicago truncatulaArbuscular mycorrhiza[SDV] Life Sciences [q-bio]expression profilingDNA microarrayBiotechnologyBioengineeringComputational biologyBiologySensitivity and Specificity03 medical and health sciencesComplementary DNABotanySymbiosisLeghemoglobin030304 developmental biologyGene Expression ProfilingfungiReproducibility of Resultsbiology.organism_classificationEquipment Failure AnalysisGene expression profilingphosphate transportercDNA array010606 plant biology & botany
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Deciphering the molecular dialogue between arbuscular mycorrhizal partners during early root interactions

2009

International audience

[SDV] Life Sciences [q-bio][SDE] Environmental Sciences[SDV]Life Sciences [q-bio][SDE]Environmental Sciencesarbuscular mycorrhizaldeciphiring the molecular dialogueroot interactionComputingMilieux_MISCELLANEOUS
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Towards a cell-specific expression atlas of arbuscular mycorrhizal roots in the model legume Medicago truncatula

2007

International audience

[SDV] Life Sciences [q-bio]ARBUSCULAR MYCORRHIZAL[SDV]Life Sciences [q-bio]MODEL LEGUMETRANSCRIPTOMEMEDICAGO TRUNCATULAComputingMilieux_MISCELLANEOUS
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