Differentiative pathway activated by 3-aminobenzamide in human osteosarcoma MG-63 cells involves FAK and PAK activities.

Polymerase Chain Reaction (PCR)

Immuno-Fluorescence (IF) on interphase polytene chromosomes of Drosophila melanogaster

In Human Retinoblastoma Y-79 Cells 3-aminobenzamide induces Differentiation

Sterilization of macroscopic poly(l-lactic acid) porous scaffolds withdense carbon dioxide: Investigation of the spatial penetration of thetreatment and of its effect on the properties of the matrix

Abstract In this work the sterilization with dense carbon dioxide of poly( l -lactic acid) (PLLA) porous scaffolds intended for tissue engineering applications was investigated with the main objective of confirming the three-dimensional efficacy of the treatment and of analysing the scaffold properties after CO2 treatment. For this purpose the scaffold was contaminated with a conventional bacterium (Escherichia coli) and with spores (Streptomyces coelicolor), a species more fascinating and difficult to inactivate. Contamination was performed in such a way to soak the whole matrix with bacteria and spores. The effect of pressure and treatment time on the efficacy of the sterilization was eva…

Sterilization of three-dimensional tissue engineering scaffolds by supercritical carbon dioxide

Medical devices, implants or patient-care equipment that will come into intimate contact with a patient must be effectively decontaminated to prevent infection or disease transmission. Non-sterile devices have significant ramifications for patient morbidity and mortality and two processes must be undertaken in order to make an item acceptable for use or implantation: cleaning and disinfection (or sterilization) [1]. Sterilization is the destruction of living organisms, and must be done without damaging the material surface and without compromising the bulk material strength or biocompatibility of implantable device. Common sterilization processes include steam autoclaving, gamma irradiation…

Induction of apoptosis by 3-AB in Y79 human retinoblastoma cells, associated with upregulation of p53 and Apo-1/Fas.

Histone deacetylase inhibitors: Epigenetic drugs acting by pleiotropic apoptotic mechanism in tumor cells and highly potent in combination with other antitumor agents.

EFFECTS OF 3-AMINOBENZAMIDE ON HUMAN RETINOBLASTOMA Y79 CELLS.

Supercritical carbon dioxide induces sterilization of PLLA scaffolds contaminated by E. coli.

The common sterilization techniques are based on physical processes that involve, for example, the use of autoclaves or systems to radiation such as γ-rays that can cause a structural change of the polymer treated. Therefore, the use of supercritical carbon dioxide (scCO2) is an excellent alternative, as it does not induce any variation of biomaterials treated (Perrut M., 2012). It's a good candidate because is readily available at low cost, non-toxic and non-flammable, it has an easily accessible critical point (7.38 MPa and 304.2 K) and excellent transport properties and wettability (White A. et al., 2005). We report the development of a supercritical CO2 based process capable of steriliz…

TRANSDUCTION OF DIFFERENTIATIVE SIGNAL ACTIVATED BY 3-AMINOBENZAMIDE IN HUMAN OSTEOSARCOMA MG-63 CELLS.

HISTONE DEACETYLASE INHIBITORS SENSITIZE HEPATOMA CELLS TO TRAIL_INDUCED APOPTOSIS

WIN55,212-2, a synthetic agonist of cannabinoid receptors, sensitizes tumor cells to TRAIL-induced apoptosis.

PACLITAXEL POTENTLY INDUCES APOPTOSIS IN HUMAN RETINOBLASTOMA Y79 CELLS

“In cellule di osteosarcoma umano MG-63, il trattamento con 3-AB, inibitore della Parp, inibisce il segnale mediato da ILK/GSK-3/Beta-catenina/LEF-1”