Analysis of Trastuzumab effects in breast cancer cells in vitro

An In Vitro model to study bone metastasis.

Effects of endothelial cells on breast cancer cells in a co-culture system.

Occurrence of S100A7 in a large sample-set of breast cancer tissues

Proteomic approach to characterize homozygous Citrus clementina Hort. ex Tan. Genotypes

Clinicopathological correlations of MMP-2 and MMP-9 in breast cancer

A multiomics analysis of S100 protein family in breast cancer

The S100 gene family is the largest subfamily of calcium binding proteins of EF-hand type, expressed in tissue and cell-specific manner, acting both as intracellular regulators and extracellular mediators. There is a growing interest in the S100 proteins and their relationships with different cancers because of their involvement in a variety of biological events closely related to tumorigenesis and cancer progression. However, the collective role and the possible coordination of this group of proteins, as well as the functional implications of their expression in breast cancer (BC) is still poorly known. We previously reported a large-scale proteomic investigation performed on BC patients f…

Proteogenomic changes in differentianting U937

Proteomic modulation of breast cancer cells (8701-BC) cocultured with normal human fibroblasts.

Differential proteomics of thyroid carcinoma cell lines

Levels of circulating gelatinases and proteomic correlations in breast cancer patients.

Anti-oncogenic role of decorin: identification of new markers.

Fibroblast’s role in breast cancer: a proteomic approach

Fibroblasts are the major mesenchymal cell types committed to the matrix formation and renewal. Moreover they are the main source of paracrine factors that influence the growth of epithelial cells of neighbouring tissues. For these properties they may be involved in tumourigenesis, either by remodelling the tumor-associated extracellular matrix (ECM), and by the production of paracrine factors that influence the growth of carcinoma cells. Studies on fibroblasts associated to carcinomas have documented their phenotypic modifications, including abnormal migratory behaviour in vitro and growth factors altered expression(Schor & Schor, 2001). In addition, fibroblasts often recruit inflammat…

Proteomic Detection of Breast-Cancer Subpopulations.

Proteomic analysis of extracellular vesicles shed in vitro by MDA MB 231 breast carcinoma cells

Cross talk between tumor cells and connective tissue plays a key role in tumor progression. The communication is due to the release of signalling molecules from both tumor cells and surrounding stromal cells. Several secreted proteins lack the N-terminal signal peptides and, therefore, they are secreted by alternative unconventional processes such as secretion mechanism mediated by vesicle shedding in the extracellular matrix. Actually, a certain number of proteins, playing roles in some aspects of tumor progression, have been found in shed vesicles. For example, EMMPRIN, carried out in vesicles shed by tumor cells, stimulates matrix metalloproteinase (MMP) production in stromal fibroblasts…

Retrospective Proteomic Screening of 100 Breast Cancer Tissues.

The present investigation has been conducted on one hundred tissue fragments of breast cancer, collected and immediately cryopreserved following the surgical resection. The specimens were selected from patients with invasive ductal carcinoma of the breast, the most frequent and potentially aggressive type of mammary cancer, with the objective to increase the knowledge of breast cancer molecular markers potentially useful for clinical applications. The proteomic screening; by 2D-IPG and mass spectrometry; allowed us to identify two main classes of protein clusters: proteins expressed ubiquitously at high levels in all patients; and proteins expressed sporadically among the same patients. Wit…

Proteomic Profiling of Colon Cancer Tissues: Discovery of New Candidate Biomarkers

Colon cancer is an aggressive tumor form with a poor prognosis. This study reports a comparative proteomic analysis performed by using two-dimensional differential in-gel electrophoresis (2D-DIGE) between 26 pooled colon cancer surgical tissues and adjacent non-tumoral tissues, to identify potential target proteins correlated with carcinogenesis. The DAVID functional classification tool revealed that most of the differentially regulated proteins, acting both intracellularly and extracellularly, concur across multiple cancer steps. The identified protein classes include proteins involved in cell proliferation, apoptosis, metabolic pathways, oxidative stress, cell motility, Ras signal transdu…

The fibroblasts: co-actors in cancer.

PROTEOMIC EFFECTS INDUCED BY MICROENVIRONMENT COMPONENTS ON 8701-BC BREAST CANCER CELLS: AN UPDATED REPORT

Proteomic analysis of Herceptin-resistance breast cancer cells

Differential proteomic and phenotypic behavior of papillary and anaplastic thyroid carcinoma cell lines: an updated study

Breast cancer proteomics: from research to clinical applications.

The exponentially increasing volume of information extracted from genomic and proteomic applications on cancer, while providing new insights into molecular composition of cancer cells and tissues, imposes new challenges on data rationalization as a tool for clinically relevant biomarker discovery. Breast cancer represents the most frequent and potentially aggressive type of cancer and, moreover, it is one of the most enigmatic and poorly predictable in its evolution, likely because it includes several different forms that behave differently among patients. Current clinical parameters for breast cancer diagnosis and cure are: tumour size, axillary lymph node status, histological grading and …

S100S PROTEIN EXPRESSION IN A LARGE SAMPLE-SET OF BREAST CANCER TISSUES

S100 proteins are low molecular weight proteins ranging in size from 9 to 13 kDa. They form homo- and heterodimers and even oligomers and are expressed in tissue and cell-specific manner [1]. It is well documented, infact, that S100 proteins have a broad range of intracellular and extracellular functions. Intracellular functions include regulation of protein phosphorylation, enzyme activity, calcium homeostasis, regulation of cytoskeletal components and regulation of transcriptional factors, so they are involved in several biological processes including cell cycle regulation, cell growth, cell differentiation, and motility [2]. Extracellularly they act in a cytokine like manner through the …

Anticancer activity of biogenerated silver nanoparticles: an integrated proteomic investigation

Silver nanoparticles (AgNPs), embedded into a specific polysaccharide (EPS), were biogenerated by Klebsiella oxytoca DSM 29614 under aerobic (AgNPs-EPSaer) and anaerobic conditions (AgNPs-EPSanaer). Both AgNPs-EPS matrices were tested by MTT assay for cytotoxic activity against human breast (SKBR3 and 8701-BC) and colon (HT-29, HCT 116 and Caco-2) cancer cell lines, revealing AgNPs-EPSaer as the most active, in terms of IC50, with a more pronounced efficacy against breast cancer cell lines. Therefore, colony forming capability, morphological changes, generation of reactive oxygen species (ROS), induction of apoptosis and autophagy, inhibition of migratory and invasive capabilities and prote…

Two-dimensional electrophoresis analysis of proteins extracted from leaves of homozygous and heterozygous citrus

Analisi di cluster proteomici per l'identificazione di sottopopolazioni di pazienti con carcinoma mammario

PHENOTYPIC PROFILING OF THYROID CARCINOMA CELL LINES AND DIFFERENTIAL SECRETION OF MATRIX METALLOPROTEINASES

Evaluation of cellular response of breast cancer cells grown on distinctive collagen substrates

Extraction of Proteins From Leaves of Homozygous and Heterozygous Citrus for Two-Dimensional Electrophoresis Analysis.

LARGE-SCALE COMPARATIVE PROTEOMICS OF BREAST CANCER SURGICAL TISSUES

In Vitro Phenotypic, Genomic and Proteomic Characterization of a Cytokine-Resistant Murine β-TC3 Cell Line

Type 1 diabetes mellitus (T1DM) is caused by the selective destruction of insulin-producing β-cells. This process is mediated by cells of the immune system through release of nitric oxide, free radicals and pro-inflammatory cytokines, which induce a complex network of intracellular signalling cascades, eventually affecting the expression of genes involved in β-cell survival. The aim of our study was to investigate possible mechanisms of resistance to cytokine-induced β-cell death. To this purpose, we created a cytokine-resistant β-cell line (β-TC3R) by chronically treating the β-TC3 murine insulinoma cell line with IL-1β + IFN-γ. β-TC3R cells exhibited higher proliferation rate and resistan…

Studio dell'interattoma di PAR-6 in cellule di carcinoma mammario 8701-BC

Comparative proteomics of breast cancer surgical tissues

Phenols content and 2-D electrophoresis protein pattern: a promising tool to monitor Posidonia meadows health state

Abstract Background The endemic seagrass Posidonia oceanica (L.) Delile colonizes soft bottoms producing highly productive meadows that play a crucial role in coastal ecosystems dynamics. Human activities and natural events are responsible for a widespread meadows regression; to date the identification of "diagnostic" tools to monitor conservation status is a critical issue. In this study the feasibility of a novel tool to evaluate ecological impacts on Posidonia meadows has been tested. Quantification of a putative stress indicator, i.e. phenols content, has been coupled to 2-D electrophoretic protein analysis of rhizome samples. Results The overall expression pattern from Posidonia rhizom…

Proteomic differentiation pattern in the U937 cell line

The U937 cell line, originally established from a histiocytic lymphoma, has been widely used as a powerful in vitro model for haematological studies. These cells retain the immature cell phenotype and can be induced to differentiate by several factors, among which 12-O-tetradecanoyl-13-phorbol acetate (TPA). Fully differentiated cells acquire the adherent phenotype and exhibit various properties typical of macrophages. However, in spite of a great deal of research devoted to the U937 cellular model, the molecular basis of biological processes involved in the monocyte/macrophage differentiation remains unclear. The present study has been undertaken to contribute to this knowledge, in order t…

Proteomic Detection of Breast-Cancer Subpopulation

Gene ontology-based annotation and comparative analysis of proteins extracted from proteomics of 100 breast cancer patients.

Background: Current clinical parameters for breast cancer diagnosis and therapy are: tumour size, axillary lymph node status, histological grading and presence or absence of metastases. Prognostic/predictive properties, such as oestrogen and progesterone receptor status, and human epidermal growth factor receptor (HER-2/neu) status are currently used for therapeutic decision. Conversely, it is now emerging that the number of genetic mutations and epigenetic deregulations in cancer is far more higher than previously thought. Therefore, proteomic screening for differential protein expression in subsets of tumor samples is an essential tool for generating data bases and biomarker discovery. Th…

A proteomic cluster for osteotropic breast cancer cells

Effects of Hypoxia on 8701-BC cancer cells.

PROTEOMIC SIGNATURE OF BREAST CANCER TISSUES FOR PATIENTS STRATIFICATION

Proteomic modulation induced in fibroblasts by breast cancer cells (8701‐BC)paracrine factors.

Proteomic and genomic modulations of breast cancer cells co-cultured with endothelial cells.

Analisi comparativa tra linee cellulari di carcinoma tiroideo papillare ed anaplastico

CHARACTERIZATION OF PAR6 INTERACTORS IN 8701-BC BREAST CANCER CELL LINE

Caratterizzazione proteomica di una linea beta cellulare resistente all'effetto citochinico

Large-scale proteomic identification of S100 proteins in breast cancer tissues

Abstract Background Attempts to reduce morbidity and mortality in breast cancer is based on efforts to identify novel biomarkers to support prognosis and therapeutic choices. The present study has focussed on S100 proteins as a potentially promising group of markers in cancer development and progression. One reason of interest in this family of proteins is because the majority of the S100 genes are clustered on a region of human chromosome 1q21 that is prone to genomic rearrangements. Moreover, there is increasing evidence that S100 proteins are often up-regulated in many cancers, including breast, and this is frequently associated with tumour progression. Methods Samples of breast cancer t…

Effetti indotti da fattori diffusibili fibroblastici sull'espressione proteica di cellule di carcinoma mammario.

Cross-talk between breast cancer cells and fibroblasts in coculture system.

CIRCULATING AND TISSUE FORMS OF MMP2 AND MMP9 IN BREAST CANCER PROGRESSION

Tumor progression and metastasis represent the leading causes of cancer related death. One of the major features that may contribute to neoplastic cell dissemination is the progressive and local degradation of the extracellular matrix (ECM) surrounding the primary tumour. Degradation of the ECM requires the coordinated action of a number of enzymes produced locally by neoplastic cells and/or stromal cells. Five categories of proteinases have been implicated in the invasive process: serine, cysteine, aspartic, threonine proteinases and matrix metalloproteinases (MMPs), also known as matrixins, which play a key role as terminal effectors of the proteolytic cascade. At present 23 members of th…

Differential proteomic and phenotypic behaviour of papillary and anaplastic thyroid cell lines.

Abstract Thyroid carcinomas account for a minority of all malignant tumours but, after those of the gonads, they represent the most common forms of endocrine cancers. They include several types, among which the papillary thyroid cancer (PTC) and the anaplastic thyroid cancer (ATC) are the best known. The two hystotypes display significant biological and clinical differences: PTC is a well differentiated form of tumour with a high incidence and a good prognosis, while the ATC is less frequent but represents one of the most aggressive endocrine tumours with morphological features of an undifferentiated type. To date, as far as we know, no conclusive studies, useful to design arrays of molecul…

New protein clustering of breast cancer tissue proteomics using actin content as a cellularity indicator

In the present study, we report the comparative proteome profiles of proteins solubilized from 37 breast cancer surgical tissues, normalized for the actin content. Blood-derived proteins were excluded from the analysis. Among the tumor-derived protein spots, a large proportion (39%) was found present in all patients. These included several glycolytic enzymes, detox and heat shock proteins, members of annexin and S100 protein families, cathepsin D, and two “rare” proteins, DDAH2 involved in the angiogenesis control, and the oncogene PARK7. Other proteins, such as psoriasin, galectin1, cofilin, peroredoxins, SH3L1, and others, showed sporadic presence and high expression level, which suggests…

Comparative detection of matrix metalloproteinase-2 and -9, interleukin-6 and c-reactive protein in breast cancer serum of patients during neoadjuvant chemotherapy

Fibroblast’s influence on breast cancer cells.

Effects of fibroblast diffusible factors on proteomic modulation of breast cancer cells.

BEHAVIOVRAL CHANGES OF BREAST CANCER CELLS IN VITRO

Differential occurrence of S100A7 in breast cancer tissues: A proteomic-based investigation

Purpose The present study reports for the first time a large-scale proteomic screening of the occurrence, subcellular localization and relative quantification of the S100A7 protein among a group of 100 patients, clinically grouped for the diagnosis of infiltrating ductal carcinoma (IDC). Experimental design To this purpose, the methods of differential proteomics, Western blotting, and immunohistochemistry were used. Results The identity of two isoforms of the protein was assessed by mass spectrometry and immunologically confirmed. Moreover, we proved by immunocytochemical applications the exclusive localization of the protein within the neoplastic cells. The correlation of S100A7 expression…

Copy Number Variants and microRNAs in Autism Spectrum Disorders: a whole-genome analysis

In recent years, there has been an increased interest by the scientific community on Autism Spectrum Disorders (ASDs), neurodevelopmental disorders of childhood with an incidence of about 1/160 children [1]. Different studies have indicated a strong genetic basis for autism susceptibility, also supported by the presence of autistic features in several monogenic disorders (e.g.,Fragile X syndrome, Tuberous sclerosis). Since 2007 Copy Number Variants (CNVs) were recognized as important genetic factors in ASD [2]. Studies performed so far have highlighted the pathogenic role of CNVs in terms of dosage change for protein-coding genes and few works have suggested the potential involvement of miR…

Breast cancer cells exhibit selective modulation induced by different collagen substrates.

During the invasive phase of malignant tumors, neoplastic cells break into the basal lamina and enter in contact with the underlying connective tissue, which concurrently undergoes extensive modifications. The aim of our present minireview is to focus the changes in the collagenous matrix occurring during breast cancer progression and to explore the possible effects of different collagen substrates on breast cancer cell behavior and proteomic modulation.

Contenuto in fenoli e profilo proteomico: un promettente strumento diagnostico per il monitoraggio delle praterie di Posidonia oceanica (L.) Delile.

Proteomic modulation of neoplastic cells by extracellular matrix components

DIFFERENTIAL PROTEOMIC ANALYSIS OF THYROID CARCINOMA CELL LlNES

Herceptin-resistance in breast cancer cells: a proteomic study.

HER-2 is a cell membrane protein that belongs to the ErbB family of receptor tyrosine kinases (HER-1, HER-2, HER-3, HER-4). The over-expression of HER-2, which results in the 25-30% of breast cancer patients, is considered a predictive and prognostic marker for breast cancer malignancy and invasiveness and makes HER-2 an excellent therapeutic target. In the last years new therapeutic strategies have been improved in order to better deal tumor diseases an to minimize collateral effects due to classic chemotherapy in patients. In this way, a new approach was the somministration of humanized antibodies directed against tumor-associated molecular targets. Among these ones Herceptin, an anti-neo…

Proteomic detection of S100 proteins in breastcancer tissues

Urinary Proteomic Profile Of A Multiple Myeloma Affected Patient

New insight on genomic and proteomic profiling of decorin-transfected breast cancer cells.

LARGE-SCALE SCREENING OF MATRIX METALLOPROTEINASES IN BREAST CANCER PATIENTS AND CLINICAL-MOLECULAR CORRELATIONS

Cytoskeleton and Proteomic Changes Induced by Fibroblasts on Breast Cancer Cells

Assessing the impact of copy number variants on miRNA genes in autism by Monte Carlo simulation.

Autism Spectrum Disorders (ASDs) are childhood neurodevelopmental disorders with complex genetic origins. Previous studies have investigated the role of de novo Copy Number Variants (CNVs) and microRNAs as important but distinct etiological factors in ASD. We developed a novel computational procedure to assess the potential pathogenic role of microRNA genes overlapping de novo CNVs in ASD patients. Here we show that for chromosomes # 1, 2 and 22 the actual number of miRNA loci affected by de novo CNVs in patients was found significantly higher than that estimated by Monte Carlo simulation of random CNV events. Out of 24 miRNA genes over-represented in CNVs from these three chromosomes only …

Herceptin-resistance in breast cancer cells: a proteomic approach

Proteomic profile of hypoxic breast cancer cells

Large-scale comparative proteomics of breast surgical tissues

IDENTIFICATION OF TYPE V COLLAGEN-BINDING PROTEINS IN 8701-BC BREAST CANCER CELLS

Proteomica comparata di un frammento chirurgico di carcinoma del colon e della metastasi epatica associata

Circulating levels of Matrix Metalloproteinases (MMP-2 and MMP-9), CRP and IL-6 during therapeutic treatment of patients with breast cancer

PHENOTYPIC PROFILING OF OSTEOTROPIC BREAST CANCER CELLS

One of the preferred locations of metastases from breast cancer is the bone tissue. On the other hand, it should be recalled that mammary tumors with equal clinical diagnosis have a different course, and also different metastatic progression. Therefore, it would be helpful to have appropriate markers of osteotropism to test on the surgical cancer tissues, in order to predict the possible propensity of the breast cancer to generate bone metastases and to adequate the therapeutic plan. We previously reported1,2 on the setting-up of an in vitro model for the study of the osteotropic propensity of breast cancer cells and the influences exerted by the bone microenvironment on the cancer cells ph…

Proteomic profiling of 13 paired ductal infiltrating breast carcinomas and non-tumoral adjacent counterparts.

According to recent statistics, breast cancer remains one of the leading causes of death among women in Western countries. Breast cancer is a complex and heterogeneous disease, presently classified into several subtypes according to their cellular origin. Among breast cancer histotypes, infiltrating ductal carcinoma represents the most common and potentially aggressive form. Despite the current progress achieved in early cancer detection and treatment, including the new generation of molecular therapies, there is still need for identification of multiparametric biomarkers capable of discriminating between cancer subtypes and predicting cancer progression for personalized therapies. One esta…

S-100 calcium binding proteins as potential markers for breast cancer metastasis.

The S-100 family of calcium-binding proteins includes about 20 members of low molecular weight characterized by two consecutive EF hands domains. They make interactions with cellular target proteins in a calcium-dependent manner; therefore they are thought to regulate a variety of physiological functions, such as cell proliferation, signal transduction, cell adhesion, motility as well as cancer metastasis.

Comparative Proteomics of Surgical Fragments of Colorectal Cancer with Non-tumoral Mucosa and Associated Liver Metastasis

Permissive and restrictive influences from breast cancer stroma

The turn-over of extracellular matrix is a physiological process, that in normal conditions and in wound healing respond to spatial and temporal regulatory mechanisms, involving several cell-matrix interaction pathways. Profound changes occur both at cellular and extracellular level, during the progression of various forms of invasive carcinomas. Collagen alterations and cellular effects. The ultrastructural and biochemical analyses of the collagenous stroma of invasive ductal breast carcinoma have demonstrated the occurrence of extensive fragmentation of pre-existing collagen fibrils and new deposition of thinner fibrils formed mostly by 1(I)3 homotrimer collagen of type I [1-3], which app…

PROTEOMIC MODULATION IN BREAST CANCER CELLS INDUCED BY MICROENVIRONMENT COMPONENTS

Differential protein expression in cytokine-sensitive and -resistant pancreatic beta cell lines

Proteomic Pattern and Phenols content: a Promising Tool to Monitor Posidonia Meadows Health State.

AUTOANTIBODIES IDENTIFICATION IN BREAST CANCER SERA BY PROTEOMIC APPROACH

Proteo-genomic modulations induced on breast cancer cells by endothelial cells

PROTEOMIC SIGNATURE OF HUMAN PRIMARY METASTATIC COLON CANCER AND ITS METASTASES TO LIVER

EFFETTI DEL TRASTUZUMAB SUL PROTEOMA DI CELLULE DI CARCINOMA MAMMARIO OVER-ESPRIMENTI HER-2

Fibroblasts enhance proliferation and invasion of Breast Cancer Cells (8701-BC)

A combined determination of circulating gelatinases and inflammatory markers in breast cancer patients during therapeutic treatment

COMPARATIVE PROFILING BY PROTEOMICS AND ZYMOGRAPHIC ACTIVITIES OF TUMORAL AND NON TUMORAL CELL LINES

The extracellular matrix (ECM) underlying epithelial tissues is involved in the maintenance of cell polarity and homeostasis. ECM is a dynamic structure under the regulated remodeling of its components. The major enzymes responsible of matrix degradation are the matrix metalloproteinases (MMPs), a well known family of zinc-dependent endopeptidases. Much attention has been focused on MMP-2 and MMP-9 because of their ability to degrade type IV collagen, a major constituent of basement membranes. A deregulated proteolysis of ECM molecules may cause the alteration of cell polarity and may contribute to the disruption of cell–cell and cell–ECM adhesions, promoting cancer progression. These alter…

Proteomic discovery of new differentiation markers in the U937 cell line

The challenge of tumor microenvironment

Multiple changes induced by fibroblasts on breast cancer cells.

It is now widely recognised that the cross-talk between cancer and stromal cells may play a crucial role in cancer progression. However little is known about the complex underlying molecular mechanisms that occur within the tumor microenvironment. Fibroblasts are the major stromal cells with multiple roles, especially towards both the extracellular matrix and the neighbouring cell population, including neoplastic cells. Consequently, proteomic analyses would provide a wider resource for a better understanding of the potential modulating effects exerted by fibroblasts on cancer cells. In this report we describe the effects of fibroblast stimulation on the breast cancer cell line (8701-BC) pr…

Hypoxia influence on differential gene expression of 8701-BC cancer cells.