Synergistic effects of neurons and astrocytes on the differentiation of brain capillary endothelial cells in culture

Brain capillary endothelial cells form a functional barrier between blood and brain, based on the existence of tight junctions that limit paracellular permeability. Occludin is one of the major transmembrane proteins of tight junctions and its peripheral localization gives indication of tight junction formation. We previously reported that RBE4.B cells (brain capillary endothelial cells), cultured on collagen IV, synthesize occludin and correctly localize it at the cell periphery only when cocultured with neurons. In the present study, we describe a three-cell type-culture system that allowed us to analyze the combined effects of neurons and astrocytes on differentiation of brain capillary …

EFFECTS OF THYROID HORMONES ON TWO PUTATIVE RNA-BINDING PROTEINS EXPRESSED IN DEVELOPING RAT BRAIN.

EFFECTS OF THYROID HORMONES ON RNA-BINDING PROTEINS EXPRESSED IN DEVELOPING RAT BRAIN

EFFETTO DEGLI ORMONI TIROIDEI SULL’ESPRESSIONE DELLE PROTEINE PIPPIN ED LPI NEL CERVELLO DI RATTO IN SVILUPPO.

ROLE OF THYROID HORMONES IN DEVELOPING RAT BRAIN AND IN CULTURED NEURONS

Thyroid hormones induce sumoylation of the cold shock domain-containing protein PIPPin in developing rat brain and in cultured neurons.

We previously identified a cold shock domain (CSD)-containing protein (PIPPin), expressed at high level in brain cells. PIPPin has the potential to undergo different post-translational modifications and might be a good candidate to regulate the synthesis of specific proteins in response to extracellular stimuli. Here we report the effects of thyroid hormone (T3) on PIPPin expression in developing rat brain. We found that a significant difference among euthyroid- and hypothyroid- newborn rats concerns sumoylation of nuclear PIPPin, that is abolished by hypothyroidism. Moreover, T3-dependence of PIPPin sumoylation has been confirmed in cortical neurons purified from brain cortices and culture…

PIPPIN SUMOYLATION IS CONTROLLED BY THYROID HORMONES

REGULATION OF THE EXPRESSION OF RNA-BINDING PROTEINS IN DEVELOPING AND ADULT RAT BRAINS: ROLE OF THYROID HORMONES