0000000001180569
AUTHOR
Emilio Sánchez Ortiga
Off-axis Digital Holographic Microscopy: practical design parameters for operating at diffraction limit
The utilization of microscope objectives (MOs) in digital holographic microscopy (DHM) has associated effects that are not present in conventional optical microscopy. The remaining phase curvature, which can ruin the quantitative phase imaging, is the most evident and analyzed. As phase imaging is considered, this interest has made possible the development of different methods of overcoming its undesired consequences. Additionally to the effects in phase imaging, there exist a set of less obvious conditions that have to be accounted for as MOs are utilized in DHM to achieve diffraction-limit operation. These conditions have to be considered even in the case in which only amplitude or intens…
Técnicas de microscopía para la mejora en resolución, obtención de seccionado óptico y medida cuantitativa de fase
La microscopía óptica convencional alcanzó el límite en resolución que predice la difracción por medio del empleo de sofisticados objetivos de microscopio, que consiguen la captura de la información espacial en el espacio de la muestra con un ángulo de captura de prácticamente 70º en aire, esto es, aperturas numéricas (NA) de 0.9 (y mayores si existe un medio de inmersión). Existe la posibilidad de sobrepasar dicho límite o, mejor dicho, encontrar otro límite en resolución siempre que el microscopio que se emplee no pueda entenderse como un microscopio convencional. Por otro lado, los microscopios convencionales carecen de capacidad de seccionado óptico ya que, cuando se tiene una muestra t…
Enhancing spatial resolution in digital holographic microscopy by biprism structured illumination
In a recent Letter by Seo et al. [Opt. Lett. 37, 4976 (2012)], the numerical correction of the quadratic phase distortion introduced by the microscope objective in digital holographic microscopy (DHM) has been presented. In this comment, we would like to draw to the attention of the authors and the readers in general that this approach could not be the optimal solution for maintaining the accuracy of the quantitative phase via DHM. We recall that the use of telecentric imaging systems in DHM simplifies the numerical processing of the phase images and produces more accurate measurements.