Performance of porous silica layers in open-tubular columns for liquid chromatography

Abstract Progress has been made in the preparation of porous silica layers in fused-silica capillaries for open-tubular liquid chromatography. The porous silica layer is prepared by (i) static coating of the silica precursor, polyethoxysiloxane (PES), followed by (ii) converting the PES film into a porous silica layer with ammonia solution. The porous silica layer can be easily modified by silane reagents commonly used in packed column high-performance liquid chromatography. The performance of the silica layer with the different phase systems was tested with polyaromatic hydrocarbons and derivatized amino acids as samples.