0000000001194366
AUTHOR
B. Bourrat
Changes in O2 Consumption of Multicellular Spheroids During Development of Necrosis
Multicellular spheroids are spherical aggregates of cells that are supplied by diffusion of oxygen and substrates from the surrounding growth medium (Sutherland and Durand, 1976). Metabolic waste products are removed from the cells in these aggregates by diffusion into the growth medium. Cells within multicellular spheroids may be exposed to environmental conditions similar to those in tissue located between nutritive microvessels. Thus, tumor spheroids make it possible to study the impact of the tumor-specific micromilieu on cellular metabolism, cell cycle state, cellular viability or response to treatment. Factors in the microenvironment of tumor cells which may be relevant in this regard…
Oxygen Consuming Regions in EMT60/Ro Multicellular Tumour Spheroids Determined by Nonlinear Regression Analysis of Experimental PO2 Profiles
Malignant cells can be studied in vitro, in a tumour-like microenvironment, by growing multicellular tumour spheroids in culture (Sutherland, McCredie and Inch, 1971). Franko and Sutherland (1979) utilized diffusion theory to explain the viable rim thicknesses of spheroids measured histologically. Without PO2 profiles, however, an unequivocal interpretation of their results was not possible. Systematic studies of the PO2 profiles in spheroids have since been made with oxygen microelectrodes by several groups (Carlsson et al., 1979; Kaufman et al., 1981; Mueller-Klieser and Sutherland, 1982a,b). Based on these measurements, new analyses utilizing diffusion theory are being developed to chara…