0000000001198476
AUTHOR
Brigitte Darchy
Réponse de quelques espèces modèles à des inhibiteurs de l'acétolactate synthase utilisés seuls ou cumulés
National audience
β-Amyrin Synthase1 Controls the Accumulation of the Major Saponins Present in Pea (Pisum sativum)
Abstract The use of pulses as ingredients for the production of food products rich in plant proteins is increasing. However, protein fractions prepared from pea or other pulses contain significant amounts of saponins, glycosylated triterpenes that can impart an undesirable bitter taste when used as an ingredient in foodstuffs. In this article, we describe the identification and characterization of a gene involved in saponin biosynthesis during pea seed development, by screening mutants obtained from two Pisum sativum TILLING (Targeting Induced Local Lesions IN Genomes) populations in two different genetic backgrounds. The mutations studied are located in a gene designated PsBAS1 (β-amyrin s…
La génétique au service du goût : caractérisation de mutants pour l'accumulation de saponines et l'activité lipoxygènase dans la graines de pois (Pisum sativum)
International audience
TILLING resources in pea: a valuable tool for functional validation
Prod 2019-88t BAP GEAPSI INRA; National audience; Since 2004, two INRA teams (UMR Agroecology Dijon and IJBP, Versailles), both partners of PEAMUST, have created 3 EMS-induced mutant collections in the framework of European or French ANR projects. The genotypes chosen for these collections have specific agronomic traits according to the research subject. For example, the Terese rms3 and -rms4 branched genotypes (Rameau et al., 1997) are well suited to the study of shoot architecture in relation with strigolactone and the lines easy to grow and to backcross in glasshouse conditions. Caméor, an early cultivar with high seed protein content, is the reference genotype for the pea sequencing pro…