Effects of soil fauna on leaching of nitrogen and phosphorus from experimental systems simulating coniferous forest floor

Long-term experiments (97–98 weeks) were carried out in macrocosm systems simulating the complexity of coniferous forest soil. The macrocosms were partially sterilized by freezing, thawing and drying, then re-inoculated with microbes alone or microbes + soil fauna. Removable microcosms containing birch litter, spruce litter, or humus were inserted into the substrate humus in the macrocosms. Two experiments used organic matter only, and in the third there was mineral soil below the humus. The macrocosms were incubated in climate chambers that simulated both summer and winter conditions. At 4- to 6-week intervals the substrates were irrigated for analyses of pH, total N, NH 4 + −N, NO 3 − −N,…