Ecdysone and ecdysterone in physogastric termite queens and eggs of Macrotermes bellicosus and Macrotermes subhyalinus

Abstract Physogastric queens and freshly laid eggs of two species of termites ( Macrotermes bellicosus and Macrotermes subhyalinus ) are found to contain high levels of ecdysteroids (molting hormones) as indicated by radioimmunoassay and Musca bioassay. Ecdysteroids are accumulated in the ovaries of the queen and then stored in the eggs since newly laid eggs contain ecdysteroid concentrations similar to those found in the ovaries. Gas chromatography-mass spectrometry demonstrates that ecdysone (α-ecdysone) as well as ecdysterone (β-ecdysone) are present in queen ovaries and in eggs and that ecdysone is quantitatively the more important ecdysteroid in both ovary and eggs.