0000000001235068

AUTHOR

L. Thomassin

showing 2 related works from this author

Apoptosis induced in vascular smooth muscle cells by oxidative stress is partly prevented by pretreatment with CGRP.

2003

MAPK/ERK pathwaymedicine.medical_specialtyVascular smooth muscleMitogen-Activated Protein Kinase 3Calcitonin Gene-Related PeptideNeuropeptideApoptosisCalcitonin gene-related peptidemedicine.disease_causeGeneral Biochemistry Genetics and Molecular BiologyMuscle Smooth VascularHistory and Philosophy of ScienceInternal medicinemedicine.arterymedicineAnimalsRats WistarAortaCells CulturedMitogen-Activated Protein Kinase 1AortaMitogen-Activated Protein Kinase 3ChemistryGeneral NeuroscienceRatsEnzyme ActivationOxidative StressEndocrinologyApoptosisMitogen-Activated Protein KinasesOxidative stressAnnals of the New York Academy of Sciences
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Calcitonin gene-related peptide partly protects cultured smooth muscle cells from apoptosis induced by an oxidative stress via activation of ERK1/2 M…

2003

Abstract Oxidative stress induced by a glucose/glucose oxidase (G/GO) generator system dose-dependently decreased the viability of cultured vascular smooth muscle cells (VSMC) as estimated by MTT assay. Cell death was induced in 40% of cells exposed to 0.2 IU/ml of the free radical generating mixture. Annexin-V labeling, Hoechst staining together with DNA laddering demonstrated that apoptosis was responsible for this cell loss. Pretreatment of the cells with 10−8 M calcitonin gene-related peptide (CGRP) significantly attenuated the damaging effect of the oxidative stress. Indeed, cell viability was estimated to be 80% in CGRP-treated group, instead of 60% in absence of CGRP treatment. This …

Programmed cell deathVascular smooth musclep38 mitogen-activated protein kinasesCalcitonin Gene-Related PeptideMyocytes Smooth MuscleApoptosisBiologyDNA ladderingCalcitonin gene-related peptidemedicine.disease_causeProtective AgentsMuscle Smooth VascularmedicineAnimalsHumansCGRPViability assayRats WistarMolecular BiologyCells CulturedMitogen-Activated Protein Kinase 3integumentary systemSAPKCell BiologyHydrogen PeroxideMAPKMolecular biologyRatsUp-RegulationNeuropeptideOxidative StressMitogen-activated protein kinaseVascular smooth muscle cellbiology.proteinMitogen-Activated Protein KinasesOxidative stressReceptors Calcitonin Gene-Related PeptideSignal TransductionBiochimica et biophysica acta
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