Author: E. Semon

0000000001235679

AUTHOR

E. Semon

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Isolation and characterisation of Nocardioides sp. SP12, an atrazine-degrading bacterial strain possessing the gene trzN from bulk- and maize rhizosp…

2003

International audience; We report the characterisation of Nocardioides sp. SP12, an atrazine-degrading bacteria isolated from atrazine-treated bulk- and maize rhizosphere soil. Based on 16S rDNA alignment, strain SP12 showed close phylogenic relationships with Nocardioides sp. C157 and Nocardioides simplex. Internal transcribed spacer (ITS) sequences of strain SP12 were longer than those of other Nocardioides sp. and present Ala- and Ile-tRNA unlike Actinomycetales. Nocardioides sp. SP12 presents a novel atrazine catabolic pathway combining trzN with atzB and atzC. Atrazine biodegradation ends in a metabolite that co-eluted in HPLC with cyanuric acid. This metabolite shows an absorption spe…

HydrolasesmaizePlant RootsPolymerase Chain Reactionbiodegradationchemistry.chemical_compound[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologyRNA Ribosomal 16SAtrazinePhylogenySoil MicrobiologyComputingMilieux_MISCELLANEOUS0303 health sciencesRhizospherebiologyStrain (chemistry)Bacterial Typing Techniques[ SDE.MCG ] Environmental Sciences/Global ChangesBiodegradation Environmental[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyBiochemistryActinomycetalesSoil microbiologyatrazinefood.ingredient[SDE.MCG]Environmental Sciences/Global ChangesMolecular Sequence DataBulk soil[ SDV.SA.SDS ] Life Sciences [q-bio]/Agricultural sciences/Soil study[SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil studynocardioides sp.MicrobiologyDNA RibosomalZea maysMicrobiology03 medical and health sciencesfoodBacterial ProteinsActinomycetalesDNA Ribosomal SpacerGeneticsMolecular Biology[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology030304 developmental biologyBase Sequence030306 microbiologyHerbicidesnocardioides spNocardioidesbiology.organism_classificationchemistryquantitative PCRrhizosphereBacteria

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PEPhysical Sciences and Engineering
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SHSocial Sciences and Humanities
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